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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Mar 2015, 36 (2)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Long-term survival of methanogens of an anaerobic digestion sludge under starvation and temperature variation


Tae Gwan Kim, Taewoo Yi, Jung-Hee Lee and Kyung-Suk Cho*

Global Top 5 Research Programme, Department of Environmental Science and Engineering, Ewha Womans University,

11-1 Daehyun-dong, Seodaemun-gu, Seoul 120-750, Korea

*Corresponding Authors Email :



Publication Data

Paper received:

13 September 2013


Revised received:

02 May 2014



20 June 2014



To investigate starvation effect on methanogen community, two identical membrane reactors were continuously operated for 84 consecutive days, with a temperature change from 50ºC to 20ºC. Continuous feeding washed out 97% biomass from reactors during the experimental period. Quantitative PCR, using mcrA genes, indicated that the methanogen abundance decreased from 7.0×107 to 1.2×107 mcrA copies·ml-1 (volume basis) at 50 °C, and then increased to 4.4 ×107 mcrA copies·ml-1 at 20 °C (p<0.05). Correspondence analysis indicated that methanogen communities were distinctly grouped by each temperature. Canonical correspondence analysis indicated that temperature showed a significant correlation with the methanogen community composition. These results suggest that methanogens can survive for a long time (at least more than 84 days) under starvation conditions, and that temperature could be a primary factor determining the density and community of methanogens.    


 Key words


Methanogens, Population dynamics, Starvation, Temperature 



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