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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Mar 2015, 36 (2)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Isolation and characterization of Pseudomonas stutzeri and its potential application in biological nitrification


Vibha Kumari1, Gaurav Rathore1*, A.K Pandey1, U.K. Chauhan2 and W.S. Lakra3

1National Bureau of Fish Genetic Resources, Lucknow - 226 002, India

2Department of Environmental Sciences, A.P.S. University, Rewa - 486 003, India

3Central Institute of Fisheries Education, Versova, Mumbai - 400 061, India

*Corresponding Author E-mail: 


Publication Data

Paper received:

09 April 2013


Revised received:

28 January 2014



03 March 2014



Ammonia-oxidizing bacteria (AOB) were isolated from sediment samples of fishponds with an aim to use them for application in biological nitrification of water. Isolation of AOB was done in an inorganic medium and nitrite-producing bacterial isolates were selected. These isolates were further screened by polymerase chain reaction using specific primers for AOB. Out of 119 nitrate positive isolates, only 12 showed positive amplification and yielded a PCR product of ~465bp. Treatment of aquaculture pond and river water with one of the bacterial isolate (HC-5) resulted in lowering of soluble ammonia level from 3.50 to 0.05 mgl-l and 7.5 to 0.01 mgl-l, respectively. Partial 16S rRNA gene sequencing of isolate HC-5 identified the microorganism as Pseudomonas stutzeri.  


 Key words

Ammonia-oxidizing bacteria, Aquaculture ponds, Biological nitrification, Pseudomonas stutzeri 



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