Journal of Environmental Biology

Isolation and characterization of Pseudomonas stutzeri and its potential application in biological nitrification

Vibha Kumari¹, Gaurav Rathore¹*, A.K Pandey¹, U.K. Chauhan² and W.S. Lakra³

¹National Bureau of Fish Genetic Resources, Lucknow - 226 002, India

²Department of Environmental Sciences, A.P.S. University, Rewa - 486 003, India

³Central Institute of Fisheries Education, Versova, Mumbai - 400 061, India

*Corresponding Author E-mail: rathore69@rediffmail.com 

Publication Data

Paper received:

09 April 2013

Revised received:

28 January 2014

Accepted:

03 March 2014

Abstract

Ammonia-oxidizing bacteria (AOB) were isolated from sediment samples of fishponds with an aim to use them for application in biological nitrification of water. Isolation of AOB was done in an inorganic medium and nitrite-producing bacterial isolates were selected. These isolates were further screened by polymerase chain reaction using specific primers for AOB. Out of 119 nitrate positive isolates, only 12 showed positive amplification and yielded a PCR product of ~465bp. Treatment of aquaculture pond and river water with one of the bacterial isolate (HC-5) resulted in lowering of soluble ammonia level from 3.50 to 0.05 mgl^-l and 7.5 to 0.01 mgl^-l, respectively. Partial 16S rRNA gene sequencing of isolate HC-5 identified the microorganism as Pseudomonas stutzeri. ? 

 Key words

Ammonia-oxidizing bacteria, Aquaculture ponds, Biological nitrification, Pseudomonas stutzeri 

Copyright ? 2015 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).