Journal of Environmental Biology

Optimization, purification and characterization of pectinases from pectinolytic strain, Aspergillus foetidus MTCC 10559

Sunil Kumar*¹, Narender K. Jain², Kailash C. Sharma², Ranjeet Paswan³, Brijesh K. Mishra⁴, Ramakrishnan Srinivasan⁵

and Shiwani Mandhania⁶

¹Central Institute of Post Harvest Engineering and Technology, Abohar-152 116, India

²Maharana Pratap University of Agriculture and Technology, Udaipur?313 001, India

³Rajendra Memorial Research Institute of Medical Sciences, Patna-800 007, India

⁴NRC on Seed Spices, Ajmer ? 305 001, India

⁵Indian Grassland and Fodder Research Institute, Jhansi ? 284 003, India

⁶CCS Haryana Agricultural University, Hisar ? 125 004, India

*Corresponding Authors Email : sunilsaini2007@gmail.com

Publication Data

Paper received:

18 September 2013

Revised received:

22 February 2014

Accepted:

21 May 2014

Abstract

A strain (MPUAT-2), isolated from coconut hull and identified as Aspergillus foetidus MTCC 10559, was used for pectinase production. Optimum pectinase production was obtained at pH 8.0 and temperature 35?C under static conditions in submerged fermentation after 5 days of incubation. Orange peel, a by-product of fruit industry, was used as a sole carbon source (3% w/v) to produce high pectinase, thus making the process cost effective. The culture filtrate was analyzed for pectin methyl esterase (PME) and endo-polygalacturonase (endo-PG) enzymes. The enzymes, PME and endo-PG were purified using ammonium sulphate precipitation and molecular exclusion chromatography (Sephadex G-75) with corresponding recovery of 39.3 and 44.3%. The partially purified enzymes were also characterized for their kinetic properties. ??   

 Key words

Aspergillus foetidus MTCC 10559,Hanes plot, Molecular exclusion chromatography, Pectinases, Submerged fermentation 

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