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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Mar 2015, 36 (2)                                     Back

nstantaneous and historical temperature effects on a-pinene

Optimization, purification and characterization of pectinases from pectinolytic strain, Aspergillus foetidus MTCC 10559



Sunil Kumar*1, Narender K. Jain2, Kailash C. Sharma2, Ranjeet Paswan3, Brijesh K. Mishra4, Ramakrishnan Srinivasan5

and Shiwani Mandhania6

1Central Institute of Post Harvest Engineering and Technology, Abohar-152 116, India

2Maharana Pratap University of Agriculture and Technology, Udaipur?313 001, India

3Rajendra Memorial Research Institute of Medical Sciences, Patna-800 007, India

4NRC on Seed Spices, Ajmer ? 305 001, India

5Indian Grassland and Fodder Research Institute, Jhansi ? 284 003, India

6CCS Haryana Agricultural University, Hisar ? 125 004, India

*Corresponding Authors Email :



Publication Data

Paper received:

18 September 2013


Revised received:

22 February 2014



21 May 2014




A strain (MPUAT-2), isolated from coconut hull and identified as Aspergillus foetidus MTCC 10559, was used for pectinase production. Optimum pectinase production was obtained at pH 8.0 and temperature 35?C under static conditions in submerged fermentation after 5 days of incubation. Orange peel, a by-product of fruit industry, was used as a sole carbon source (3% w/v) to produce high pectinase, thus making the process cost effective. The culture filtrate was analyzed for pectin methyl esterase (PME) and endo-polygalacturonase (endo-PG) enzymes. The enzymes, PME and endo-PG were purified using ammonium sulphate precipitation and molecular exclusion chromatography (Sephadex G-75) with corresponding recovery of 39.3 and 44.3%. The partially purified enzymes were also characterized for their kinetic properties. ??   



 Key words

Aspergillus foetidus MTCC 10559,Hanes plot, Molecular exclusion chromatography, Pectinases, Submerged fermentation 



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