Journal of Environmental Biology

Genetic diversity of wild and cultivated genotypes of pigeonpea through RAPD and SSR markers

Babasaheb C. Walunjkar*, Akarsh Parihar, Nirbhay Kumar Singh and L.D. Parmar

C.P. College of Agriculture, S.D. Agricultural University, S.K. Nagar-385 506, India

*Corresponding Author E-mail: baba.walunjkar@gmail.com

Publication Data

Paper received:

14 June 2013

Revised received:

22 December 2013

Accepted:

22 February 2014

Abstract

Eight wild and four cultivated pigeonpea genotypes were subjected to RAPD and microsatellite analysis, with 40 primers each. Out of these, eight RAPD and five SSR primers were found polymorphic. RAPD primers showed 100% polymorphism and produced a total of 517 DNA fragments, whereas SSR primers produced 67 fragments and they too showed 100% polymorphism. The RAPD markers revealed highest similarity co-efficient of 0.93 (GT-100 and ICPL-87), whereas the highest similarity co-efficient obtained with SSR markers was 1.00 (GTH-1 and GT-100). Average PIC value obtained with RAPD and SSR were 0.90 and 0.18, respectively. The arithmetic mean heterozygosity and marker index were 0.90 and 22.47 respectively with RAPD marker, whereas the corresponding values for SSR markers were 0.18 and 33.66. Moreover, the four wild genotypes (Cajanus scarabaeoides, Rhyncosia rufescence, Cajanus cajanifolius and Rhyncosia canna) and the four cultivars (GTH-1, GT-100, ICPL-87 and GT-1) grouped distinctly in the same subgroups of the dendrograms obtained with both RAPD and SSR analysis. Therefore, the findings of SSR supplement and validate the results obtained with RAPD analysis. ??   

 Key words

Cluster analysis, Fingerprinting, Microsatellites, Random primers, Redgram 

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