Journal of Environmental Biology

Isolation and characterization of Flavobacterium columnare from freshwater ornamental goldfish Carassius auratus

Dev Kumar Verma¹, Gaurav Rathore², Pravata Kumar Pradhan¹*, Neeraj Sood¹ and Peyush Punia¹

?1ICAR- National Bureau of Fish Genetic Resources, Lucknow - 226 002, India

²ICAR- Central Institute of Fisheries Education, Versova, Mumbai - 400 061, India

*Corresponding Author E-mail: pradhanpk1@gmail.com

Publication Data

Paper received:

02 November 2013

Revised received:

18 February 2014

Accepted:

20 February 2014

Abstract

Filamentous bacteria overlaying ulcerated area on the body surface were observed in the wet-mout preparation from a moribund goldfish with saddle back appearance. The causative agent was identified as Flavobacterium columnrae, on the basis of biochemical test, species - specific polymerase chain reaction (PCR) and sequencing of 16S rDNA gene with the universal bacterial primers. Furthermore, the strain (ING-1) attributed to genomovar II in 16S rDNA PCR-restriction fragment length polymorphism (PCR-RFLP) and sequence analysis. In phylogenetic analysis, the strain ING-1, produced typical columnaris disease symptoms in rohu (Labeo rohita) fingerlings within 10 days. This is a new record about molecular detection and identification of Flavobacterium columnare, occurring naturally? on a new host Carassius auratus in India. ?   

 Key words

Bioassay, Carassius auratus, Flavobacterium columnare, Genomovar, PCR-RFLP 

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