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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Mar 2015, 36 (2)                                     Back

nstantaneous and historical temperature effects on a-pinene

Isolation and characterization of Flavobacterium columnare from freshwater ornamental goldfish Carassius auratus



Dev Kumar Verma1, Gaurav Rathore2, Pravata Kumar Pradhan1*, Neeraj Sood1 and Peyush Punia1

?1ICAR- National Bureau of Fish Genetic Resources, Lucknow - 226 002, India

2ICAR- Central Institute of Fisheries Education, Versova, Mumbai - 400 061, India

*Corresponding Author E-mail:


Publication Data

Paper received:

02 November 2013


Revised received:

18 February 2014



20 February 2014



Filamentous bacteria overlaying ulcerated area on the body surface were observed in the wet-mout preparation from a moribund goldfish with saddle back appearance. The causative agent was identified as Flavobacterium columnrae, on the basis of biochemical test, species - specific polymerase chain reaction (PCR) and sequencing of 16S rDNA gene with the universal bacterial primers. Furthermore, the strain (ING-1) attributed to genomovar II in 16S rDNA PCR-restriction fragment length polymorphism (PCR-RFLP) and sequence analysis. In phylogenetic analysis, the strain ING-1, produced typical columnaris disease symptoms in rohu (Labeo rohita) fingerlings within 10 days. This is a new record about molecular detection and identification of Flavobacterium columnare, occurring naturally? on a new host Carassius auratus in India. ?   


 Key words

Bioassay, Carassius auratus, Flavobacterium columnare, Genomovar, PCR-RFLP 



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