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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Jan 2015, 36 (1)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Effect of lambda cyhalothrin and temephos on detoxification enzyme systems in Culex quinquefasciatus (Diptera: Culicidae)

 

R. Muthusamy and M.S. Shivakumar*

Molecular Entomology Lab, Department of Biotechnology, Periyar University, Salem-636011, India

*Corresponding Author E-mail: skentomol@gmail.com

 

 

 

 Publication Data

Paper received:

05 March 2013

 

Revised received:

25 September 2013

 

Re-revised received:

10 February 2014

 

Accepted:

24 April 2014

 

Abstract

Mosquitoes serve as vector for transmitting diseases. Among mosquitoes, Culex quinquefasciatus transmits lymphatic filariasis, yellow fever Japanese encephalitis etc. Application of chemical insecticides is still the best option for vector control programmes. Continuous use of these chemicals on mosquito reduces its effects. The present study determined the baseline susceptibility of Cx. quinquefasciatus in response to λ-cyhalothrin and temephos treatments. In addition, the biochemical mechanisms and zymogram analysis involved in insecticide detoxification among larval mosquitoes were studied. The larval bioassay indicated high LC50 value for λ-cyhalothrin (0.1484ppm) as compared to temephos (0.01092ppm). While AChE assay showed increased activity in temephos treatments, glutathione reductase (GR) and esterase levels were increased at both the treatments. Esterase quantitative analysis revealed the expression of three bands at 43kDa, 67kDa and 245kDa. The findings suggest that insensitivity of AChE, esterase and high GR activity may play an important role in developing resistance to synthetic pyrethroid and organophosphate insecticides in Cx. quinquefasciatus population. ?  

 

 Key words

 

Culex quinquefasciatus, Detoxification enzymes, Insecticide Resistance, Synthetic pyrethriod 

 

 

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