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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Jan 2015, 36 (1)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Application of response surface methodology for optimization of polygalacturonase production by Aspergillus niger



Kaushlesh K. Yadav1, Neelima Garg1*, Devendra Kumar1, Sanjay Kumar1, Achal Singh2 and M. Muthukumar3

1Division of Post Harvest Management, Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow–226 101, India

2Division of Crop Production, Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow–226 101, India

3Division of Crop Improvement and Biotechnology, Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow–226 101, India

*Corresponding Author E-mail:



Publication Data

Paper received:

05 May 2013


Revised received:

22 December 2013



03 March 2014



Polygalacturonase (PG) degrades pectin into D-galacturonic acid monomers and is used widely in food industry especially for juice clarification. In the present study, fermentation conditions for polygalacturonase production by Asgergillus niger NAIMCCF-02958, using mango peel as substrate, were optimized using the 23 factorial design with central composite rotatable experimental design (CCRD) of response surface methodology (RSM). The maximum PG activity 723.66 U g-1 was achieved under pH 4.0, temperature 30 ºC and 2% inoculum by response surface curve. The experimental value of PG activity was higher 607.65 U g-1 than the predicted value 511.75 U g-1. Under the proposed optimized conditions, the determination coefficient (R2) was equal to 0.66 indicating that the model could explain 66% of the total variation as well as establish the relationship between the variables and the responses. ANOVA analysis and the three dimensional plots also confirmed interactions among the parameters.    


 Key words


Aspergillus niger, Enzyme production, Mango peel, Polygalacturonase production, Response surface methodology 



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