isolation and culture of highly efficient psychrophilic
bacteria from oil-contaminated soils in South Korea
Van H.T. Pham1,
Jaisoo Kim1* and Seung-Woo Jeong2
Life Science, Graduate School of Kyonggi university, Suwon 443-760, South
Environmental Engineering, Kunsan National University, Kunsan 573-701, South
Authors Email : firstname.lastname@example.org
02 May 2013
28 August 2013
25 November 2013
13 January 2014
known that isolation of oil-degrading bacterial strains is difficult at low
temperatures, and the biodegradation efficiency of oil-contaminated soil is
significantly reduced in cold weather. In this study, 14 strains were
isolated from oil-contaminated soil that grew well at 10?C by using a newly
developed culture method.11 of the 14 isolates were successfully cultured in
mineral salts medium containing 1,500 ppm of oil components, 500 ppm each
kerosene, gasoline, and diesel as carbon sources, at 10?C for 2 weeks. The
oil degradation efficiencies of these 11 isolates ranged from 36% to 100%, as
measured by total petroleum hydrocarbon (TPH) degradation analyses. Three
strains (Pseudomonas simiae G1-10, P. taiwanensis Y1-4, and P.
koreensis Gwa2) displayed complete degradation (100%), and six others (P.
frederiksbergensis G2-2, P. arsenicoxydans Y2-1, P. umsongensis
Gwa3, P. migulae Gwa5, Rhodococcus jialingiae Y1-1, and R.
qingshengii Y2-2) showed relatively high degradation efficiencies
(>70%). This study suggests that these isolates can be effectively
utilised in thetreatment of oil-contaminated soil in landfarming, especially
soil, Psychrophilic, Pseudomonas, Rhodococcus, Bioremediation
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