of sophorolipid biosurfactant produced by Cryptococcus sp. VITGBN2 and
its application on Zn(II) removal from electroplating wastewater
and Nilanjana Das*
Biotechnology Division, School of Biosciences and Technology VIT University,
Vellore-632 014, India
Author E-mail: firstname.lastname@example.org
19 July 2013
20 October 2013
25 December 2013
11 March 2014
present study aimed at elucidating the role of biosurfactant produced by
yeast for the removal of Zn(II) ions from electroplating wastewater. The
yeast species isolated from CETP, Vellore, Tamilnadu was identified as Cryptococcus
sp.VITGBN2, based on molecular techniques, and was found to be potent
producer of biosurfactant in mineral salt media containing vegetable oil as
additional carbon source. Chemical structure of the purified biosurfactant
was identified as acidic diacetate sophorolipid through GC-MS analysis.
Interaction of Zn(II) ions with biosurfactant was monitored using FT-IR, SEM
and EDS analysis. Zn (II) removal at 100 mg l-1 concentration was
84.8 % compared were other synthetic surfactants (Tween 80 and sodium dodecyl
sulphate), yeast mediated biosurfactant showed enhanced Zn (II) removal in
batch mode. The role of biosurfactant on Zn(II) removal was evaluated in
column mode packed with biosurfactant entrapped in sodium alginate beads. At
a flow rate of 1 ml min-1 and bed height of 12 cm, immobilized
biosurfactant showed 94.34 % Zn(II) removal from electroplating wastewater.
The present study confirmed that Zn(II) removal was biosurfactant mediated.
This is the first report establishing the involvement of yeast mediated
biosurfactant in Zn(II) removal from wastewater.
VITGBN2, Sophorolipid, Zn (II) removal
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