Journal of Environmental Biology

Production and optimization of L-asparaginase by an actinobacterium isolated from Nizampatnam mangrove ecosystem

M. Usha Kiranmayi¹, Sudhakar Poda² and M. Vijayalakshmi¹*

¹Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur -522510, India

²Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur -522510, India

*Corresponding Author?s Email : profmvl@gmail.com

Publication Data

Paper received:

13 March 2012

Revised received:

17 August 2012

Accepted:

26 December 2013

Abstract

The aim of the present study was to isolate and screen actinomycetes from the mangrove sediments of Nizampatnam that are potent to produce L-asparaginase, an enzyme that catalyses the hydrolysis of asparagine. A total of 31 actinomycetes strains were isolated, of which 6 strains were positive for L-asparaginase. Several physico-chemical parameters were optimized for maximizing L-asparaginase production by the potent strain identified as Pseudonocardia endophytica VUK-10. Production of L-asparaginase by the strain was high in modified Asparagine glucose salts broth (FM-4) (3.96 IU/ml) as compared to other tested media. Maltose (6.99 IU ml^-1) and L-asparagine (7.42 IU ml^-1) were found to be the most suitable carbon and nitrogen sources for optimum enzyme production. Maximum production of L-asparaginase was found in the culture medium with pH 8 and temperature 30?C incubated for four days.? This is the first report on the production of L-asparaginase by Pseudonocardia endophytica VUK-10 from Nizampatnam mangrove sediments. ?

 Key words

L-asparaginase, Pseudonocardia endophytica, Optimization, Mangrove sediments

Copyright ? 2014 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).