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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Sep 2014, 35 (5)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Production and optimization of L-asparaginase by an actinobacterium isolated from Nizampatnam mangrove ecosystem

 

 

M. Usha Kiranmayi1, Sudhakar Poda2 and M. Vijayalakshmi1*

1Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur -522510, India

2Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur -522510, India

*Corresponding Author’s Email : profmvl@gmail.com

 

 

 

Publication Data

Paper received:

13 March 2012

 

Revised received:

17 August 2012

 

Accepted:

26 December 2013

 

Abstract

The aim of the present study was to isolate and screen actinomycetes from the mangrove sediments of Nizampatnam that are potent to produce L-asparaginase, an enzyme that catalyses the hydrolysis of asparagine. A total of 31 actinomycetes strains were isolated, of which 6 strains were positive for L-asparaginase. Several physico-chemical parameters were optimized for maximizing L-asparaginase production by the potent strain identified as Pseudonocardia endophytica VUK-10. Production of L-asparaginase by the strain was high in modified Asparagine glucose salts broth (FM-4) (3.96 IU/ml) as compared to other tested media. Maltose (6.99 IU ml-1) and L-asparagine (7.42 IU ml-1) were found to be the most suitable carbon and nitrogen sources for optimum enzyme production. Maximum production of L-asparaginase was found in the culture medium with pH 8 and temperature 30ºC incubated for four days.  This is the first report on the production of L-asparaginase by Pseudonocardia endophytica VUK-10 from Nizampatnam mangrove sediments.  

 

 Key words

L-asparaginase, Pseudonocardia endophytica, Optimization, Mangrove sediments

 

 

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