JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editorial Board
    Reviewer Panel
    RnD Division
    Subscription Info
    Contact Journal
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Authoring Guidelines
    Publication Process
    Track Paper Status
 
Announcements
    JEB Award
 

Search the Journal web-site through Google:


        Abstract - Issue Sep 2014, 35 (5)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Identification of simple sequence repeat markers associated with wilt resistance in pigeonpea 

 

Ganesh D. Khalekar1*, Amrapali A. Akhare2, Santosh J. Gahukar2, Nirbhay Kumar Singh1 and Mithlesh Kumar1

1C.P. College of Agriculture, S.D. Agricultural University, S.K. Nagar-385 506, India

2Biotechnology Centre, Department of Agricultural Botany, Dr. Panjabrao Deshmukh Agricultural University, Akola-444 104, India

*Corresponding Authorís Email : gkhalekar@gmail.com

 

 

 

Publication Data

Paper received:

20 May 2013

 

Revised received:

25 September 2013

 

Accepted:

11 October 2013

 

 

Abstract

The present study was carried out with the objective of identifying markers for Fusarium wilt resistance in pigeonpea using simple sequence repeat (SSR) and bulk segregant analysis (BSA). The wilt resistant (ICPL 87119) and wilt susceptible (T.Vishakha-1) genotypes were crossed and their F2 population was used for marker analysis. Although, the parents were surveyed with 76 SSR primers to identify the polymorphic markers, only 26 primers were found polymorphic between the parents under study. The polymorphic information content scores of SSR markers ranged between 0.077 to 0.333, with an average of 0.18 per marker. These 26 primers were selected for BSA, which indicated that five SSR primers (PFW 26, PFW 31, PFW 38, PFW 56, and PFW 70) were able to distinguish the resistant and susceptible bulks and parents for wilt resistance. Hence, these five SSR markers can be utilized further for identification of wilt resistant genotypes of pigeon pea and for development of new wilt resistant varieties of pigeonpea. 

 

Key words

Bulk segregant analysis, Fusarium, Pigeonpea, Simple sequence repeat, Wilt

 

Copyright © 2014 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).