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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Sep 2014, 35 (5)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Screening of novel microorganisms for biosurfactant and biocontrol activity against Phytophthora infestans

 

 

Sonica Tomar1, B.P. Singh2, Mehi Lal1*, M.A. Khan1, Touseef Hussain1, Sanjeev Sharma2, S.K. Kaushik1 and Satish Kumar3

1Central Potato Research Institute, Modipuram Campus, Meerut-250 110, India

2Central Potato Research Institute, Shimla -171 001, India

3Meerut College, C.C.S University, Meerut-250 001, India

*Corresponding Author E-mail: mehilalonline@gmail.com

 

 

 

Publication Data

Paper received:

14 March 2013

 

Revised received:

25 July 2013

 

Accepted:

05 October 2013

 

 

Abstract

In the present study, 95 isolates of bacteria were tested for their biosurfactant as well as biocontrol activity against Phytophthora infestans. The results revealed that only 15.8% isolates showed biosurfactant activity. The emulsification index ranged from 0-68% and 24.2% isolates showed positive reaction for biosurfactant properties. In emulsification assay and oil spreading test, 18.95% and 5.26% isolates, respectively scored positive for biosurfactant production. Among all, only five isolates were found effective against P. infestans, for biocontrol properties. Pseudomonas aeruginosa-1 showed 62.22% inhibition zone after 72 hrs while P. aeruginosa-3 showed 46.42%. Forty-eight hrs old culture supernatants were highly effective in food-poisoning test, tuber slice test and detached leaf method against P. infestans. In whole potato plant test, bacterial cell based formulation, culture supernatant and bacterial cell suspension of P. aeruginosa-1 showed 10.42%, 9.94% and 17.96% diseases severity respectively, as against 53.96% in control. This isolate holds promise as biological control agent against P. infestans in field. 

 

Key words

 

Bacteria, Biocontrol, Biosurfactant, Phytophthora infestans, Potato 

 

 

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