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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Sep 2014, 35 (5)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Screening of novel microorganisms for biosurfactant and biocontrol activity against Phytophthora infestans



Sonica Tomar1, B.P. Singh2, Mehi Lal1*, M.A. Khan1, Touseef Hussain1, Sanjeev Sharma2, S.K. Kaushik1 and Satish Kumar3

1Central Potato Research Institute, Modipuram Campus, Meerut-250 110, India

2Central Potato Research Institute, Shimla -171 001, India

3Meerut College, C.C.S University, Meerut-250 001, India

*Corresponding Author E-mail:




Publication Data

Paper received:

14 March 2013


Revised received:

25 July 2013



05 October 2013




In the present study, 95 isolates of bacteria were tested for their biosurfactant as well as biocontrol activity against Phytophthora infestans. The results revealed that only 15.8% isolates showed biosurfactant activity. The emulsification index ranged from 0-68% and 24.2% isolates showed positive reaction for biosurfactant properties. In emulsification assay and oil spreading test, 18.95% and 5.26% isolates, respectively scored positive for biosurfactant production. Among all, only five isolates were found effective against P. infestans, for biocontrol properties. Pseudomonas aeruginosa-1 showed 62.22% inhibition zone after 72 hrs while P. aeruginosa-3 showed 46.42%. Forty-eight hrs old culture supernatants were highly effective in food-poisoning test, tuber slice test and detached leaf method against P. infestans. In whole potato plant test, bacterial cell based formulation, culture supernatant and bacterial cell suspension of P. aeruginosa-1 showed 10.42%, 9.94% and 17.96% diseases severity respectively, as against 53.96% in control. This isolate holds promise as biological control agent against P. infestans in field. 


Key words


Bacteria, Biocontrol, Biosurfactant, Phytophthora infestans, Potato 



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