Journal of Environmental Biology

Development of bioformulation and delivery system of

Pseudomonas fluorescens against bacterial leaf blight of rice (Xanthomonas oryzae pv. oryzae)

P.P.Jambhulkar¹* and? P. Sharma²

?¹Agricultural Research Station, (MPUAT, Udaipur), Banswara-327 001, India

²Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi-110 012, India

*Corresponding Author?s Email : ppjambhulkar@gmail.com?

Publication Data

Paper received:

26 February 2013

Revised received:

18 May 2013

Accepted:

19 August 2013

Abstract

Antagonistic potential of Pseudomonas fluorescens isolate RRb-11 has been evaluated against bacterial leaf blight (BLB) pathogen of rice in vitro, in vivo, microplot and field tests. RRb-11 isolate mass multiplied in substrates like talc and kaolinite powder and bran of barley, soybean and wheat to prepare suitable bioformulation. The maximum shelf life of P. fluorescens was recorded in talc based bioformulation up to 150 days after storage. In rhizosphere competence study, the root rhizosphere of talc, kaolinite and barley based bioformulation treated plants showed good survivability and competence even up to 90 days after treatment. In field study, the talc based bioformulation was applied and the best results were obtained when talc based bioformulation of P. fluorescens RRb-11 was applied as seed treatment, seedling root dip and soil application in combination which reduced the disease by 92.3 and 88.5% over control in the year 2009 and 2010, respectively. This treatment also produced maximum yield of 3.88 t ha^-1 i.e., 61% greater than control.

 Key words

Bioformulation, Pseudomonas fluorescens, Rhizosphere, Shelf life, Survivability

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