Comparison
of Ecklonia cava, Ecklonia stolonifera and
Eisenia
bicyclis for phlorotannin extraction
Muhammad Tanvir
Hossain Chowdhury1, Issa Bangoura1, Ji-Young Kang1,
Ji Young Cho2, Jin Joo3,
Yoo Seong Choi4,
Dong Soo Hwang5 and Yong-Ki Hong1*
1Department of
Biotechnology, Pukyong National University, Namku, Busan 608-737, Korea
2Department of
Marine Biotechnology, Soonchunhyang University, Asan 336-745, Korea
3Department of
Applied Chemistry, Kyungpook National University, Bukgu, Daegu 702-701, Korea
4Department of
Chemical Engineering, Chungnam National University, Yuseonggu, Daejeon
305-764, Korea
5Postech Ocean
Science & Technology Institute, Pohang University of Science &
Technology, Namgu, Pohang 790-784, Korea
*Corresponding
Author E-mail: ykhong@pknu.ac.kr
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Publication
Data
Paper received:
21 January 2013
Revised received:
08 June 2013
Accepted:
31 July 2013
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Abstract
Phlorotannins
are polyphenols of marine algae, particularly brown seaweed, having multiple
biological activities. A reverse phase-high performance liquid chromatography
method was developed for rapid and routine quantification of two major
phlorotannins, dieckol and phlorofucofuroeckol-A (PFE-A), from boiling water-
and organic solvent-extracts of brown seaweeds Ecklonia cava, E.
stolonifera and Eisenia bicyclis. The regression equations for
dieckol and PFE-A were as follows: the concentration (mg ml-1) = 16.56 ? peak
height (cm) + 0.44, and the concentration = 20.60 ? peak height (cm) + 0.11,
with correlation coefficients of 0.996 and 0.999, respectively. Compared to
organic solvent extraction, the recovery yield of dieckol from boiling water
extracts of E. cava, E. stolonifera and E. bicyclis was 86%,
93%, and 98%, respectively. The recovery yield of PFE-A was 74%, 86% and 62%,
respectively. Antioxidant activity was detected in each E. bicyclis
water extract (91%), followed by E. stolonifera (90%) and E. cava
(74%). Dieckol and PFE-A showed almost 9- and 7-fold stronger antioxidant
activity than the standard butylhydroxytoluene, and 6- and 4-fold greater
than L-ascorbic acid in molar concentration, respectively.
Key
words
Dieckol,
Ecklonia cava, Ecklonia stolonifera, Eisenia bicyclis,
Phlorofucofuroeckol-A
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