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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Jul 2014, 35 (4)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Comparison of Ecklonia cava, Ecklonia stolonifera and

Eisenia bicyclis for phlorotannin extraction


Muhammad Tanvir Hossain Chowdhury1, Issa Bangoura1, Ji-Young Kang1, Ji Young Cho2, Jin Joo3,

Yoo Seong Choi4, Dong Soo Hwang5 and Yong-Ki Hong1*

1Department of Biotechnology, Pukyong National University, Namku, Busan 608-737, Korea

2Department of Marine Biotechnology, Soonchunhyang University, Asan 336-745, Korea

3Department of Applied Chemistry, Kyungpook National University, Bukgu, Daegu 702-701, Korea

4Department of Chemical Engineering, Chungnam National University, Yuseonggu, Daejeon 305-764, Korea

5Postech Ocean Science & Technology Institute, Pohang University of Science & Technology, Namgu, Pohang 790-784, Korea

*Corresponding Author E-mail:




Publication Data

Paper received:

21 January 2013


Revised received:

08 June 2013



31 July 2013




Phlorotannins are polyphenols of marine algae, particularly brown seaweed, having multiple biological activities. A reverse phase-high performance liquid chromatography method was developed for rapid and routine quantification of two major phlorotannins, dieckol and phlorofucofuroeckol-A (PFE-A), from boiling water- and organic solvent-extracts of brown seaweeds Ecklonia cava, E. stolonifera and Eisenia bicyclis. The regression equations for dieckol and PFE-A were as follows: the concentration (mg ml-1) = 16.56 peak height (cm) + 0.44, and the concentration = 20.60 peak height (cm) + 0.11, with correlation coefficients of 0.996 and 0.999, respectively. Compared to organic solvent extraction, the recovery yield of dieckol from boiling water extracts of E. cava, E. stolonifera and E. bicyclis was 86%, 93%, and 98%, respectively. The recovery yield of PFE-A was 74%, 86% and 62%, respectively. Antioxidant activity was detected in each E. bicyclis water extract (91%), followed by E. stolonifera (90%) and E. cava (74%). Dieckol and PFE-A showed almost 9- and 7-fold stronger antioxidant activity than the standard butylhydroxytoluene, and 6- and 4-fold greater than L-ascorbic acid in molar concentration, respectively.


 Key words

Dieckol, Ecklonia cava, Ecklonia stolonifera, Eisenia bicyclis, Phlorofucofuroeckol-A 


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