potential of wild mustard (Cleome viscosa) and
(Tribulus terrestris) against mosquito vectors in
semi-arid region of Western Rajasthan
Karam V. Singh and Sapna Sharma
Research Centre (ICMR), New Pali Road, Jodhpur - 342 005, India
Author E-mail: firstname.lastname@example.org
12 December 2012
29 May 2013
31 July 2013
viscosa L. (Family: Capparaceae) commonly known as Tickweed or wild
mustard and Tribulus terrestris L. (Family: Zygophyllaceae) commonly
known as Gokhru, growing wildly in the desert areas in the monsoon and post
monsoon season, are of great medicinal importance. Comparative larvicidal
efficacy of the extracts from seeds of C. viscosa and fruits and
leaves of T. terrestris was evaluated against 3rd or early
4th stage larvae of Anopheles stephensi (Liston), Aedes
aegypti (Linnaeus) and Culex quinquefasciatus (Say) in different organic
solvents. 24 and 48hr LC50 and LC90 values along with
their 95% fiducial limits, regression equation, chi-square (χ2)/
heterogeneity of the response was determined by log probit regression
analysis. The 24hr LC50 values as determined for seeds of C.
viscosa were 144.1, 99.5 and 127.1 (methanol); 106.3, 138.9 and 118.5
(acetone) and 166.4, 162.5 and 301.9 mg l-1 (petroleum ether
extracts) for all the three mosquito species respectively showing that
methanol and acetone extracts were a little bit more effective than the
petroleum ether extracts. Experiments were carried out with fruits and leaves
of T. terrestris with all the solvents and mosquito species. The 24hr
LC50 values, as determined for fruits of T. terrestris were
70.8,103.4 and 268.2 (methanol); 74.0,120.5 and 132.0 (acetone) and 73.8,
113.5 and 137.4 mg l-1 (petroleum ether extracts) while the 24hr
LC50 values for leaves were 124.3, 196.8 and 246.5 (methanol);
163.4, 196.9 and 224.3 (acetone) and 135.8,176.8 and 185.9 mg l-1
(petroleum ether extracts) for all the three mosquito species respectively.
The results clearly indicate that fruit extracts of T. terrestris
were more effective as compared to leaves extracts in the three solvents
tested. Larvae of An. stephensi were found more sensitive to both
fruit and leaves extracts of T. terrestris followed by larvae of Ae.
aegypti and Cx. quinquefasciatus. Extracts from the seeds of C.
viscosa were found less effective as compared to the fruit extracts of T.
terrestris indicating that active larvicidal principle may be present in
the fruits of this plant species. The study would be of great importance
while formulating the control strategy, for vectors of malaria, dengue and
lymphatic filariasis, based on alternative plant based insecticides in this
viscosa, Mosquito larvicides, Semi-arid region, T. terrestris
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