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Abstract - Issue Mar 2014, 35 (2) Back
nstantaneous and historical temperature effects on a-pinene
and immunohistochemical identification of Flavobacterium psychrophilum
from the tissue of catfish, Clarias batrachus
Vinay Verma and
Biotechnology and Fish Pathology Laboratory, Department of Animal Science, M.
J. P. Rohilkhand University, Bareilly ?243 006, India
Author E-mail: email@example.com
20 November 2012
15 May 2013
22 July 2013
study deals with the identification of bacteria in paraffin embedded tissue
of Indian catfish, Clarias batrachus experimentally infected with F.
psychrophilum to ascertain it's precise role in the occurrence and state
of carrier stage of coldwater disease in asymptomatic fishes. Significant (P
< 0.05) mortality was recorded in C. batrachus of this region due
to persistent occurrence of creamish/yellowish with non smooth edged colonies
of rod shaped flexible gram negative bacterium, F. psychrophillum.
Serodiagnosis made through polyclonal antiserum dilutions (raised against H
antigen of Aqb Fb ? 6 isolate in rabbit) revealed that staining intensity of
this bacterium was dilution dependent as tissue and smears treated with 1:300
and 1:500 antiserum dilutions conferred close relationship at
immunofluorescence level with F. psychrophilum and stained deeply in
the tissues and smear prepared from liver, kidney and intestine of experimentally
infected C. batrachus. Result of this study would be helpful in
identifying the fish pathogenic bacteria in carrier fish where mortality
occurs without explicit clinical signs of disease.
batrachus, Flavobacterium psychrophilum,? Immunofluorescence,
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