JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editor in Chief
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
    JEB Awards

Google Search the Journal web-site:

    Abstract - Issue Mar 2014, 35 (2)                                     Back

nstantaneous and historical temperature effects on a-pinene

Profiling of the dichloromethane-induced

proteome expression changes


Seul-Ki Park and Mi-Young Lee*

Department of Medical Biotechnology, SoonChunHyang University, Asan, Chungnam 336 600, Korea

*Corresponding Author E-mail:




Publication Data

Paper received:

25 May 2012


Revised received:

08 November 2012



24 December 2012



A colorless volatile liquid dichloromethane (DCM) is used as solvents in chemical manufacturing processes. The major route of exposure is via inhalation and to a lesser extent through the skin and digestive tract. We investigated the effects of DCM on rats and analyzed their liver proteome expression changes. Approximately 1,100 protein spots that were detected by 2-dimensional gel electrophoresis showed reproducible abundance. Mass spectrometry based proteomics was used to characterize the changes in the liver proteome in response to DCM exposure. Consequently, 7 of these spots showed significant changes in expression level after DCM treatment. These proteins were 3 paralogues of glutathione S-transferase, beta 1 globin, 2 hemoglobin beta-2 and alpha-2 globulin. Of these, the expression of alpha-2 globulin was also confirmed by western blot. The differential expression of these proteins might be caused by DCM exposure.



 Key words

Dichloromethane, Liver protein, Proteomics, Rat



Copyright ? 2014 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).