Journal of Environmental Biology

Studies on antibiotic resistant activity of Pseudomonas sp., isolated from fresh water loach, Lepidocephalichthys guntea and water sample of river Lotchka, Darjeeling, India

Rudra Prasad Roy¹, Min Bahadur² and Sudip Barat^1*

¹Aquaculture and Limnology Research Unit, Department of Zoology, University of North Bengal, Siliguri-734 013, India

²Genetics and Molecular Biology? Laboratory, Department of Zoology, University of North Bengal, Siliguri-734 013, India

*Corresponding Author E-mail: sudipbarat@rediffmail.com

Publication Data

Paper received:

17 August 2012

Revised received:

10 February 2013

Accepted:

05 June 2013

Abstract

An investigation was conducted in the isolates of Pseudomonas sp. from skin, gill and gut of fresh water resident loach, Lepidocephalichthys guntea (Hamilton Buchanan) and water sampled from four different sites of River Lotchka in Darjeeling district, West Bengal, India to study the antibiotic resistance status. Isolated bacteria were identified by different biochemical procedures and Polymerase Chain Reaction using genus specific 16S rDNA primers for confirmation of identification of Pseudomonas sp. Antibiotic susceptibility test of bacterial isolates was also done by Disc Diffusion method. Pseudomonas sp. found in the river water ranged from 1.7x 10⁶ cfu ml l^-1 to 2.6 x 10⁶ cfu ml l^-1 and gut of fish1.2 x 10⁶ cfu g^-1.? A total of 40 Pseudomonas sp. were isolated from skin, gill and gut of fish and four sites of the river. Maximum resistance was exhibited for ampicillin, penicillin-G and cephalothin (>57, 100 and 100% resistant, respectively in the four different sites of river and body parts of fish). For ciprofloxacin, gentamycin and streptomycin resistance was almost nil. Presence of Pseudomonas sp. resistance to multiple antibiotics indicated that the river water and resident fish were contaminated with multi-antibiotic resistant enteric pathogenic bacteria.

 Key words

Antibiotic resistance, Fresh water loach, L. guntea, Pseudomonas sp

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