JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editorial Board
    Reviewer Panel
    RnD Division
    Subscription Info
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Authoring Guidelines
    Publication Process
    Track Paper Status

Search the Journal web-site through Google:

        Abstract - Issue Jan 2014, 35 (1)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Molecular characterization of Fusarium moniliforme var.

subglutinans isolates


Pradeep Kumar1,4*, V. K. Gupta2, A.K. Misra3 and D. R. Modi4

1The Department of Biotechnology Engineering, Ben Gurion University of The Negev, Be'er Sheva-84105, Israel

2Molecular Glycobiotechnology Group, National University of Ireland Galway-353, Ireland

3Discipline of Biochemistry,Central Institute for Subtropical Horticulture, Lucknow-227 017, India

4Department of Biotechnology, BBA University, Lucknow- 226 025, India

*Corresponding Author E-mail:




Publication Data

Paper received:

21 July 2012


Revised received:

09 January 2013



27 February 2013




Molecular characterization of 42 isolates of mango (Mangifera indica L.) malformation pathogen Fusarium moniliforme var. subglutinans collected from different agro-climatic zones of India were performed using RAPD markers. Twenty RAPD primers of OPA series were selected for screening of which; six primers OPA-1, 3, 7, 8, 9 and 18 were found to be clearly discriminates and differentiated the F. moniliforme var. subglutinans isolates and showed reproducible banding patterns. A sum total of 598 bands were generated from these random primers having range from 4.0% to 90.9% of similarity among the 42 isolates of F. moniliforme var. subglutinans. The data were used to construct a dendrogram with unweighted pair group method of arithmetical averages (UPGMA) algorithm, using the NTSYS-software. Dendogram revealed more than 90% similarity among all the 42 isolates which shows that all isolates approximately have same genetic identity. Hence, in the current research findings RAPD proved a reproducible and tractable means of differentiating F. moniliforme var. subglutinans isolates.


 Key words

F. moniliforme, RAPD markers, Malformation, Mango, PCR, Variability 



Copyright 2014 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).