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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Nov 2013, 34 (6)                                     Back


nstantaneous and historical temperature effects on a-pinene

Genotoxic effect of cadmium in okra seedlings: Comperative investigation with population parameters and molecular markers

 

Semra Soydam Aydin1, Esin Basaran2, Demet Cansaran-Duman3?and S?mer Aras2*

1Department of Plant and Animal Production, Ulukisla Vocational School University of Nigde, Ulukisla, Nigde, 51900, Turkey

2Biotechnology Section, Department of Biology, Faculty of Science, University of Ankara, Tandogan, Ankara, 06100, Turkey

3Biotechnology Institute, University of Ankara, Tandogan, Ankara, 06100, Turkey?

*Corresponding Author email : aras@science.ankara.edu.tr

 

 

 

 Publication Data

Paper received:

11 April 2012

 

Revised received:

26 December 2012

 

Accepted:

25 January 2013

 

Abstract

Plants are considered as good bioindicators because of their significant role in food chain transfer. They are also easy to grow, adaptable to environmental stresses and can be used for assaying a range of environmental conditions in different habitats. Thus, many plant species have been used as bioindicators. In order to evaluate the genotoxic effect of cadmium, okra (Abelmoschus esculantus L.) seedlings were treated with different concentrations (30, 60, 120 mg l-1) of cadmium and investigated for their population parameters such as inhibition of root growth; total soluble protein content, dry weight and also the impact of metal on the genetic material by RAPD analysis. Root growth and total soluble protein content in okra seedlings were reduced with increased Cd concentrations. RAPD analysis indicated formation of new bands mostly at 60 and 120 mg l-1?Cd treatments. Altered DNA band patterns and population parameters after Cd treatments suggest that okra could be used as an indicator to reveal the effects of genotoxic agents.

 

Key words

Cadmium, DNA damage, Genotoxicity, Okra, RAPD,

 

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