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Abstract - Issue Nov 2013, 34 (6) Back
nstantaneous and historical temperature effects on a-pinene
Characterization
of a sodium dodecyl sulphate-degrading
Pseudomonas
sp. strain DRY15 from Antarctic soil
M.I.E.
Halmi1, W.S.W. Hussin1, A. Aqlima1, M.A. Syed1, L. Ruberto2, W.P. MacCormack2?and M.Y. Shukor1*
1Department of Biochemistry,
Faculty of Biotechnology and Biomolecular Sciences, University Putra
Malaysia
43400 UPM Serdang, Selangor, Malaysia
2lnstituto Antartico
Argentino, Cerrito 1248 (1010), Buenos Aires, Argentina
*Corresponding
Author email : yunus @biotech.upm.edu.my
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Publication
Data
Paper received:
04 December 2012
Revised received:
07 May 2013
Accepted:
01 June 2013
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Abstract
A
bacterium capable of biodegrading surfactant sodium dodecyl sulphate (SDS)
was isolated from Antarctic soil. The isolate was tentatively identified as Pseudomonas
sp. strain DRY15 based on carbon utilization profiles using Biolog GN plates
and partial 16S rDNA molecular phylogeny. Growth characteristic studies
showed that the bacterium grew optimally at 10?C, 7.25 pH, 1 g l-1?SDS as a sole carbon source
and 2 g l-1?ammonium
sulphate as nitrogen source. Growth was completely inhibited at 5 g l-1?SDS. At a tolerable initial
concentration of 2 g l-1,
approximately 90% of SDS was degraded after an incubation period of eight
days. The best growth kinetic model to fit experimental data was the Haldane
model of substrate inhibition with a correlation coefficient value of 0.97.
The maximum growth rate was 0.372 hr-1?while the saturation constant or half velocity constant
(Ks) and inhibition constant (Ki), were 0.094% and 11.212 % SDS,
respectively. Other detergent tested as carbon sources at 1 g l-1?was Tergitol NP9, Tergitol
15S9, Witconol 2301 (methyl oleate), sodium dodecylbenzene sulfonate (SDBS),
benzethonium chloride, and benzalkonium chloride showed Tergitol NP9, Tergitol
15S9, Witconol 2301 and the anionic SDBS supported growth with the highest
growth exhibited by SDBS.
Key words
Antarctica,
Biodegradation, Pseudomonas sp., SDS
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