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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Nov 2013, 34 (6)                                     Back


nstantaneous and historical temperature effects on a-pinene

Characterization of a sodium dodecyl sulphate-degrading

Pseudomonas sp. strain DRY15 from Antarctic soil

 

M.I.E. Halmi1, W.S.W. Hussin1, A. Aqlima1, M.A. Syed1, L. Ruberto2, W.P. MacCormack2?and M.Y. Shukor1*

1Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra

Malaysia 43400 UPM Serdang, Selangor, Malaysia

2lnstituto Antartico Argentino, Cerrito 1248 (1010), Buenos Aires, Argentina

*Corresponding Author email : yunus @biotech.upm.edu.my

 

 

 

 

Publication Data

Paper received:

04 December 2012

 

Revised received:

07 May 2013

 

Accepted:

01 June 2013

 

Abstract

A bacterium capable of biodegrading surfactant sodium dodecyl sulphate (SDS) was isolated from Antarctic soil. The isolate was tentatively identified as Pseudomonas sp. strain DRY15 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. Growth characteristic studies showed that the bacterium grew optimally at 10?C, 7.25 pH, 1 g l-1?SDS as a sole carbon source and 2 g l-1?ammonium sulphate as nitrogen source. Growth was completely inhibited at 5 g l-1?SDS. At a tolerable initial concentration of 2 g l-1, approximately 90% of SDS was degraded after an incubation period of eight days. The best growth kinetic model to fit experimental data was the Haldane model of substrate inhibition with a correlation coefficient value of 0.97. The maximum growth rate was 0.372 hr-1?while the saturation constant or half velocity constant (Ks) and inhibition constant (Ki), were 0.094% and 11.212 % SDS, respectively. Other detergent tested as carbon sources at 1 g l-1?was Tergitol NP9, Tergitol 15S9, Witconol 2301 (methyl oleate), sodium dodecylbenzene sulfonate (SDBS), benzethonium chloride, and benzalkonium chloride showed Tergitol NP9, Tergitol 15S9, Witconol 2301 and the anionic SDBS supported growth with the highest growth exhibited by SDBS.

 

Key words

Antarctica, Biodegradation, Pseudomonas sp., SDS

 

 

 

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