Journal of Environmental Biology

Impact of osmotic stress and temperature on pigments and

proteins of Anabaena strains

 

Ruchi Sharma¹*, O. P. Chahar², Monica Bhatnagar³ and Ashish Bhatnagar³

¹Department of Biotechnology, S.D.M. PG Girls College, Bhilwara- 311 001, India

²Department of Biotechnology, JJT University, Jhunjhunu- 333 001, India

³Department of Biotechnology, M.D.S University, Ajmer -305 009, India

*Corresponding Author email : ruchi.vic@gmail.com

 

 

 

Publication Data

Paper received:

19 April 2012

 

Revised received:

25 September 2012

 

Accepted:

20 October 2012

 

Abstract

A study on Anabaena strains was carried out to investigate the effect of combined stress of polyethylene glycol 6000 (0,-5 bar and -7 bar) and temperature (300C and 45 0C) on photosynthetic pigments (chlorophyll a, carotenoids, phycobilins) and total proteins as stress metabolites. The selected strains, A. oryzae and A. ellipsospora were sensitive to osmotic stress at ambient temperature of 30 0C and increase in the temperature to 45 0C was harmful to the growth of Anabaena strains. Chlorophyll a contents decreased at 30 0C and -7 bar pressure from 8.868 to 0.710 µg ml^-1 and 4.360 to 0.220 µg ml^-1 in A. oryzae and in A. ellipsospora, respectively and at -7 bar osmotic stress and 45 0C temperature, decrease in Chl a content of A.oryzae was 92.9%, however A. ellipsospora was highly sensitive and could not survive under these conditions. Carotenoids and phycobilins also showed decreasing trends with increase in temperature and osmotic potential. Moreover, combined stress adversely depleted the cellular activities leading to a marked decrease in total protein contents of the cell. A.oryzae and A.ellipsospora showed varying tolerance potential to osmotic and temperature stresses. The results indicated that A. ellipsospora was more sensitive towards these stresses in comparison to A.oryzae.

 

Key words

Anabaena strains, Osmotic stress, Photosynthetic pigments

 

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