Journal of Environmental Biology

Phylogenetic relationship and molecular identification of five

Indian Mahseer species using COI sequence

Jyoti Sati¹, Seema Sah¹, Himani Pandey², Shahnawaz Ali², Prabhati Kumari Sahoo²,
Veena Pande¹?and Ashoktaru Barat²*

¹Department of Biotechnology, Kumaun University, Nainital-263 136, India

²Molecular Genetics Laboratory, Directorate of Coldwater Fisheries Research, Bhimtal-263 136, India.

*Corresponding Author email : abarat58@hotmail.com

Publication Data

Paper received:

26 September 2012

Revised received:

10 December 2012

Accepted:

20 February 2013

Abstract

This study examined the phylogenetic relationship and? identification of five Mahseer species (Tor putitora, Tor tor, Tor khudree, Tor chelynoides and Neolissochilus hexagonolopis) using partial sequencing of a Cytochrome Oxidase I (COI) DNA barcodes. The sequence analysis data showed that 134 (21.61%) sites out of 628 sites were variable without insertion or deletion.? Rate of transition (70.5%) were higher than transversion (29.41%). There was a high inter-species divergence (range 4.1% to 12.2%) in Mahseer species as compared to intra-specific sequence divergence (1.7% for T.? putitora, 1.2% for T. tor, 1.4% for T. khudree, 3.0% for T. chelynoides, 0.26 % for N. hexagonolopis). The phylogenetic tree, constructed by maximum parsimony, maximum likelihood and unweighted pair group average methods revealed similar results suggesting that T. putitora, T. tor and T. khudree had a close relationship to each other while maximum divergence was observed in T. chelynoides, which was also confirmed by the genetic distance data. The results indicate that COI sequencing or bar-coding is useful in unravelling phylogenetic relationship and identification of Mahseer species.

Key words

Bar-coding, Cytochrome Oxidase I, Genetic distance, Mahseer, Phylogenetic relationship

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