Journal of Environmental Biology

Degradation of isoprothiolane by a defined microbial
consortium using response surface methodology

A. Arul Selvi¹, Navin K. Rastogi²?and Haravey K. Manonmani¹*

¹Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute (CFTRI),
Mysore 570 020, India

²Department of Food Engineering, Central Food Technological Research Institute (CFTRI), Mysore-570 020, India

*Corresponding Author email : manonmani_99@yahoo.com

Publication Data

Paper received:

15 October 2011

Revised received:

01 February 2012

Re-revised received:

20 July 2012

Accepted:

27 July 2012

Abstract

A defined microbial consortium was developed for the degradation of isoprothiolane. Isoprothiolane-biodegradation parameters were optimized using Response Surface Methodology (RSM). Three variables chosen for the study were inoculum concentration (50?1500 ?g protein ml^-1), temperature (25?35oC) and pH (4?8) each at levels -1.682, -1, 0, 1 and 1.682. Incubation time of 72 hr was kept constant. Degradation of different concentrations of isoprothiolane was studied. The optimized conditions obtained were, inoculum concentration of 50 ?g protein ml^-1?at 30oC and pH between 4?8. The maximum predicted percentage degradation of 100, 100, 100, 100 and 95.5 was obtained respectively for 5, 10, 20, 30 and 50 ppm of initial isoprothiolane concentrations at different pH levels 7.7, 6.8, 6.2, 4.7 and 4.6. Validation of the model indicated that experimental values were found to be in agreement with the predicted one.

Key words

Degradation, Isoprothiolane, Microbial consortium, Response surface methodology

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