JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editorial Board
    Reviewer Panel
    RnD Division
    Subscription Info
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Authoring Guidelines
    Publication Process
    Track Paper Status

Search the Journal web-site through Google:

        Abstract - Issue Mar 2013, 34 (2)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Impact of alphamethrin on biochemical parameters of

Channa punctatus


G. Tripathi* and H. Singh

Department of Zoology, J.N.V. University, Jodhpur-342 033, India

*Corresponding Author email :






Publication Data

Paper received:

08 February 2011


Revised received:

15 April 2012



26 July 2012



Impact of alphamethrin (synthetic pyrethroid) on profiles of lactate dehydrogenase (LDH), catalase (CAT), DNA, RNA and protein in liver, brain, gill and skeletal muscle of the freshwater food fish Channa punctatus were investigated. Exposure of sublethal concentration of alphamethrin (0.018 ppm for 14 days) increased the activity of LDH in liver (1.8 fold), brain (1.4 fold), gill(1.6 fold), and skeletal muscle(2.2 fold) of the fish. However, it significantly decreased the activity of CAT in the tissues of liver (54%), skeletal muscle (52%), gill (51%) and brain (49%) of the fish. Similarly, DNA (skeletal muscle (36%), liver (30%), brain (28%) and gill (25%)) RNA (liver (42%), brain (32%), gill (35%) and skeletal muscle (45%) ) and protein content (45%), brain (42%), gill (36%), and skeletal muscle (27%)) declined in different tissues of the fish exposed to alphamethrin. Maximum increase in the level of LDH was in skeletal muscle (2.2 fold) and minimum in brain (1.4 fold). Maximum reduction in CAT profile was in liver (54%), and minimum in brain (49%). Declines in DNA was maximum in skeletal muscle (36%) and minimum in gill (25%) whereas RNA and protein content were maximum in liver (42% and 45% respectively) and minimum in skeletal muscle (45% and 27% respectively). Alphamethrin was toxic to the freshwater fish due to its inducing effect on anaerobic enzyme (LDH) and inhibitory effect on antioxidant enzyme (CAT), DNA, RNA and protein. This reflected alphamethrin associated increase in anaerobiosis and decrease in oxidative defense and impairment in protein synthesizing capacity of C. punctatus. Further, induction in LDH and reduction in CAT and protein profile may be used as biomarker of alphamethrin toxicity in fish.



Key words

Alphamethrin, Channa punctatus, Antioxidative enzyme, Anaerobic enzyme


Copyright 2013 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).