Journal of Environmental Biology

Effects of fluoride on germination, early growth and
antioxidant enzyme activities of legume plant species

Prosopis juliflora

Poonam Saini, Suphiya Khan, Mamta Baunthiyal and Vinay Sharma*

Department of Bioscience and Biotechnology, Banasthali University, Banasthali Vidyapith-304 022, India

*Corresponding Author email : vinaysharma30@yahoo.co.uk

Publication Data

Paper received:

22 September 2011

Revised received:

03 May 2012

Accepted:

26 May 2012

Abstract

Prosopis juliflora (Mimosoideae) is a fast growing and drought resistant tree of semi-arid region of India where fluoride (F) toxicity is a common problem. In the present investigations this species was fluoride tested to check their capacity as bioindicator plant and its efficiency to accumulate. To achieve this aim, P. juliflora seedlings grown in hydroponic culture containing different concentrations of F were analyzed for germination percentage together with some biochemical parameters viz, antioxidant enzyme activities, total chlorophyll and accumulation of F in different plant parts. After 15 days of treatment, root growth (r = -0.928, p<0.01), shoot growth (r = -0.976, p<0.01), vigor index (r = -0.984, p<0.01) were in decreasing trend with increasing concentration of NaF. Both catalase (3.2 folds) and peroxidase (2.7 folds) enzymes activity increased with increase in F concentration. Plant accumulated larger portion of the F in the roots (1024.63 ?g g^-1?d.wt.) followed by shoot (492.30 ?g g^-1?d.wt.). As P. juliflora did not show any morphological changes (marginal and tip chlorosis of leaf portions, necrosis and together these features are referred to as leaf ?tip-burn?) therefore, this species may be used as suitable bioindicator species for potentially F affected areas. Further, higher accumulation of F in roots indicates that P. juliflora is a suitable species for the removal of F in phytoremediation purposes.

Key words

Fluoride toxicity, Antioxidants, Prosopis juliflora, Bioindicator

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