Google Search the Journal web-site:
|
Abstract - Issue Nov 2012, 33 (6) Back
nstantaneous and historical temperature effects on a-pinene
Utilization of
intron-flanking EST-specific markers in the genetic characterization of Artemisia
annua genotypes from the
trans-Himalayan
region of Ladakh, India
Author
Details
|
Jitendra Kumar
|
Defence Institute of High Altitude
Research, Defence R & D Organization, Leh-194 101, India
|
Prasad Bajaj
|
Department of Biotechnology and
Bioinformatics, Jaypee University of Information Technology,
Solan-173 234, India
|
Harvinder Singh
|
Department
of Biotechnology and Bioinformatics, Jaypee University of Information
Technology,
Solan-173
234, India
|
Gyan
P. Mishra
|
Defence
Institute of High Altitude Research, Defence R & D Organization, Leh -
194 101, India
|
Ravi
B. Srivastava
|
Defence
Institute of High Altitude Research, Defence R & D Organization, Leh -
194 101, India
|
Pradeep
K. Naik
|
Department of Biotechnology
and Bioinformatics, Jaypee University of Information Technology,
Solan-173 234, India
e-mail : pknaik73@rediffmail.com; pradeep.naik@juit.ac.in
|
Publication
Data
Paper received:
16 February 2010
Revised received:
04 August 2011
Accepted:
21 September 2011
|
Abstract
Genetic variation was assessed utilizing intron-flanking
EST-specific markers among genotypes of Artemesia annua collected from
two sampling sites viz. Nubra (9,600 ft) and Leh (11,500 ft) valleys
of the trans-Himalayan region, Ladakh, India. The available ESTs (3,60,906)
sequences of A. annua were aligned with the genomic sequences of Arabidopsis
to developed ?intron-flanking? EST-PCR based primers. These primers
anneal with the conserved region of exon (flanking to the intron) and
amplified the introns. Out of the 39 primers selected and tested on 20
genotypes of A. annua, we successfully exploited 81 codominant intron
length polymorphism (ILP) markers, with an average of 2.08 markers per primer
and 92.04% polymorphism detection. Clustering of genotypes revealed
distribution of genotypes into 2 distinct clusters with respect to their site
of collection. Significantly, this study demonstrates that Arabidopsis
genome sequence can be useful in developing gene-specific PCR-based markers
for other non-model plant species like A. annua in the absence of
genome sequences.
Key words
Artemisia annua, Intron length polymorphism,
Molecular marker, Genome characterization
|
Copyright
? 2012 Triveni Enterprises. All rights reserved. No part of the Journal can
be reproduced in any form without prior permission. Responsibility
regarding the authenticity of the data, and the acceptability of the
conclusions enforced or derived, rest completely with the author(s).
|
|