JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Home
    Editor in Chief
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
 
Announcements
    JEB Awards
 

Google Search the Journal web-site:


    Abstract - Issue Sep 2012, 33 (5)                                     Back


nstantaneous and historical temperature effects on a-pinene

Effects of tert-butyl hydroperoxide on Ca2+ ATPase activity in isolated

rat hepatocytes and its reversal by antioxidants

 

Author Details

 

Sangram Singh

(Corresponding author)

Department of Biochemistry, Dr. R.M.L. Avadh University, Faizabad-224001, India ?????????????????

e-mail : sangram_rml@yahoo.co.in

Richa Agarwal

Department of Biochemistry, Dr. R.M.L. Avadh University, Faizabad-224001, India

Farrukh Jamal

Department of Biochemistry, Dr. R.M.L. Avadh University, Faizabad-224001, India

Sudhir Mehrotra

Department of Biochemistry, University of Lucknow , Lucknow-226007, India ?????

Rakesh Singh

Department of Biochemistry, Banaras Hindu University , Varanasi-221104, India

 

 

 

 

Publication Data

Paper received:

27 November 2010

 

Revised received:

16 May 2011

 

Accepted:

11 June 2011

 

Abstract

Calcium ions play an important role in various physiological processes such as nerve impulse transmission, muscle contraction, hormone action, blood clotting. They ions act as an intracellular second messenger, relaying information within cells to regulate their activity. To understand the mechanism of hepatotoxicity of t-BHP, studies were carried out using freshly isolated rat hepatocytes. The effect of t-BHP on Ca2+ accumulation and Ca2+ uptake by rat hepatocytes was monitored using 45Ca2+. It caused decrease in 15% accumulation of 45Ca2+ in comparison to the control group. t-BHP also significantly decreased the Ca2+ ATPase activity in isolated hepatocytes. This decrease in Ca2+ATPase activity by t-BHP was reversed 40% by naturally occurring antioxidant glutathione (GSH) and 20% by the synthetic antioxidant butylated hydroxy toluene (BHT). These results indicate that the hepatotoxic action of t- BHP involves oxidative stress as evident by the protection accorded by various antioxidants employed in the study as well as impairment of intracellular calcium homeostasis which can lead to liver cell injury.

 

Key words

Oxidative stress, Reactive oxygen species, Hepatotoxicity, Tert-butyl hydroperoxide, Calcium homeostasis.

 

Copyright ? 2012 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).