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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Sep 2012, 33 (5)                                     Back

nstantaneous and historical temperature effects on a-pinene

Effects of tert-butyl hydroperoxide on Ca2+ ATPase activity in isolated

rat hepatocytes and its reversal by antioxidants


Author Details


Sangram Singh

(Corresponding author)

Department of Biochemistry, Dr. R.M.L. Avadh University, Faizabad-224001, India ?????????????????

e-mail :

Richa Agarwal

Department of Biochemistry, Dr. R.M.L. Avadh University, Faizabad-224001, India

Farrukh Jamal

Department of Biochemistry, Dr. R.M.L. Avadh University, Faizabad-224001, India

Sudhir Mehrotra

Department of Biochemistry, University of Lucknow , Lucknow-226007, India ?????

Rakesh Singh

Department of Biochemistry, Banaras Hindu University , Varanasi-221104, India





Publication Data

Paper received:

27 November 2010


Revised received:

16 May 2011



11 June 2011



Calcium ions play an important role in various physiological processes such as nerve impulse transmission, muscle contraction, hormone action, blood clotting. They ions act as an intracellular second messenger, relaying information within cells to regulate their activity. To understand the mechanism of hepatotoxicity of t-BHP, studies were carried out using freshly isolated rat hepatocytes. The effect of t-BHP on Ca2+ accumulation and Ca2+ uptake by rat hepatocytes was monitored using 45Ca2+. It caused decrease in 15% accumulation of 45Ca2+ in comparison to the control group. t-BHP also significantly decreased the Ca2+ ATPase activity in isolated hepatocytes. This decrease in Ca2+ATPase activity by t-BHP was reversed 40% by naturally occurring antioxidant glutathione (GSH) and 20% by the synthetic antioxidant butylated hydroxy toluene (BHT). These results indicate that the hepatotoxic action of t- BHP involves oxidative stress as evident by the protection accorded by various antioxidants employed in the study as well as impairment of intracellular calcium homeostasis which can lead to liver cell injury.


Key words

Oxidative stress, Reactive oxygen species, Hepatotoxicity, Tert-butyl hydroperoxide, Calcium homeostasis.


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