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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Sep 2012, 33 (5)                                     Back

nstantaneous and historical temperature effects on a-pinene

Studies on strains of Trichoderma spp. from high altitude of††††

Garhwal Himalayan region


Author Details


B. B.† Joshi

(Corresponding author)

Indian† Institute† of† Sugarcane† Research,† Lucknow-226002, India

e-mail :†

M. P. Vishwakarma

Department† of† Botany,† HNB Garhwal† University,† Srinagar, Garhwal-246174, India

D. Bahukhandi

Division of Seed Technology, Indian Grassland and Fodder Research Institute, Jhansi-284003, India

R. P. Bhatt

Department† of† Botany,† HNB Garhwal† University,† Srinagar, Garhwal-246174, India



Publication Data

Paper received:

08 February 2010


Revised received:

06 September 2011



18 October 2011



Two species of Trichoderma i.e. T. harzianum and T. viride have been isolated from the soil samples collected from the higher altitude (2000-3500 m) of Garhwal Himalayan region in India. The two species were grown in Petri plates on TSM agar media and it was also observed that the optimum temperature and pH for Trichoderma growth was 300 C and 5.5 respectively. When incubated on TSM agar medium at 40 C, the fungus grew normally with heavy induced sporulation within three weeks of incubation. Induction of sporulation on exposure to low temperature appeared to be strategies for survival of these species in extreme cold environment temperature 4 to 50 C. Antifungal activities of the two species of Trichoderma were demonstrated with phytopathogenic fungi in dual cultures. The antifungal metabolites produced by Trichoderma spp., diffusible as well as volatile, caused abnormalities in pathogenic fungi. Plant growth promotion of Trichoderma spp. was also shown through plant analysis in greenhouse.†


Key words

Trichoderma spp., Trichoderma selective medium, Biocontrol agent, Soil-borne pathogens


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