nstantaneous and historical temperature effects on a-pinene
Effect of fluoride pollution
on genetic diversity
of a medicinal tree, Syzygiumcumini
Author Details
Suphiya Khan
Deptt. of Bioscience and Biotechnology, Banasthali
University, BanasthaliVidyapith
- 304 022, India
MamtaBaunthiyal
Deptt. of Bioscience and Biotechnology, Banasthali
University, BanasthaliVidyapith
- 304 022, India
AlkaKumari
Deptt. of Bioscience and Biotechnology, Banasthali
University, BanasthaliVidyapith
- 304 022, India
Vinay Sharma
(Corresponding
author)
Deptt. of Bioscience and Biotechnology, Banasthali
University, BanasthaliVidyapith
- 304 022, India
e-mail:
vinaysharma30@yahoo.co.uk
Publication Data
Paper received:
13
December 2010
Revised
received:
19
April 2011
Accepted:
25 May 2011
Abstract
Syzygiumcumini Linn. (Myrtaceae)
is a medicinal tree (Jamun) used worldwide in
treatment of diabetes. However, no molecular data is available on genetic
polymorphism and its relationship, if any with fluoride pollution. In the
present study, the genetic variability of two populations of S. cumini growing in fluoride rich soils and normal
soils located in Rajasthan and Haryana regions of India, respectively was
determined using random amplified polymorphic DNA (RAPD) markers. Different
measures of diversity in Rajasthan populations: Shannon?s index of phenotypic
diversity (I) = 0.440; Nei?s genetic diversity (h)
= 0.292; effective number of alleles per locus (Ne)
= 1.497; total species diversity (Hsp) = 0.307 and
within population diversity (Hpop) = 0.158 showed
high diversity in comparison to Haryana populations. Thus, it seems that
Rajasthan population responds with increased genetic variation resulting
possibly from new mutation that affect allele frequencies as a consequence of
adaptation to contaminated environment. This may imply that the increased
diversity levels may act as a buffer to combat fluoride stress. Cluster
analysis and principal component analysis (PCA) results showed mixing between
the populations. ?
Key words
Fluoride;
Genetic diversity, RAPD, Syzygiumcumini
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