JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editorial Board
    Reviewer Panel
    RnD Division
    Subscription Info
    Contact Journal
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Authoring Guidelines
    Publication Process
    Track Paper Status
 

Search the Journal web-site through Google:


        Abstract - Issue May 2012, 33 (3)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Colonization of probiotic bacteria and its impact on

ornamental fish Puntius conchonius

 

Author Details

 

K.R. Divya

(Corresponding author)

Deptt. of Aquaculture and Fishery Microbiology, M.E.S. Ponnani College, Ponnani - 679 586, India

e-mail : divyaravi27@gmail.com

A. Isamma

Deptt. of Aquaculture and Fishery Microbiology, M.E.S. Ponnani College, Ponnani - 679 586, India

V. Ramasubramanian

Unit of Aquatic Biotechnology and Livefeed Culture, Department of Zoology, Bharathiar University, Coimbatore - 641 046, India

S. Sureshkumar

Deptt. of Aquaculture and Fishery Microbiology, M.E.S. Ponnani College, Ponnani - 679 586, India

T.S. Arunjith

Deptt. of Aquaculture and Fishery Microbiology, M.E.S. Ponnani College, Ponnani - 679586, India

 

 

Publication Data

Paper received:

23 July 2010

 

Revised received:

07 April 2011

 

Re-revised received:

06 June 2011

 

Accepted:

14 June 2011

 

Abstract

The present study was conducted to assess the establishment and effect of probiotic bacteria such as Bacillus coagulans, Bacillus mesentericus, and Bifidobacterium infantis in the gut of freshwater ornamental fish Puntius conchonius. Postlarvae of 60 days old Puntius conchonius divided in four experimental groups each with three replicates. T1, T2 and T3 groups were fed with Bacillus coagulans, Bacillus mesentericus and Bifidobacterium infantis enriched copepod Thermocyclops decipiens respectively. T0 was the control group (without probiotic treated T.decipiens). The experiment was conducted for 45 days. The initial gut analysis of fish showed significant level of pathogenic bacteria in the gut of fish (p<0.05).? Total plate count of initial gut analysis of fish larvae were enumerated as 1.2 x 104 CFU ml-1.? The bacteriological study indicated that final gut microflora of post-larvae have decreased level of pathogens. Total plate count of T1, T2, T3 and T0 were reported to be as 1.08x104, 1.06x104, 1.27x104 and 2.1x105 CFU ml-1, respectively. Three experimental groups (T1, T2 and T3) were significantly different from control group (T0) (p<0.05). At the end of the experiment, establishment of probiotics were examined. One week after probiotics administration, gut analysis of fish larvae showed, poor spore formation of Bacillus coagulans (2.3x10? CFU ml-1), when compared to B. mesentericus (3.2?0.03x10? CFU ml-1) and Bifidobacterium infantis (3.1x10? CFU ml-1). The results from the study suggest that the probiotic bacteria significantly established in gut of P. conchonius and significant effects on the pathogenic gut inhabitants of the fish. ??

 

Key words

Probiotics, Enrichment, Puntius conchonius, Thermocyclops decipiens?

 

Copyright ? 2012 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).