Department of Zoology,
Aligarh Muslim University, Aligarh-202002, India
The aim of present study was to evaluate the genotoxic effect of heavy metal in Channa
punctatus through the micronucleus test,
chromosomal aberrations and sister chromatid
exchange. The fish were kept separately in 0.5, 1.0, 2.0 and 5.0 ppm cadmium chloride for 3 days. For micronucleus test
blood was collected from caudal vein and smeared on clean slides fixed in
methanol and stained with 2% Giemsa. Mean frequency
of micronuclei observed was 0.10, 0.15, 0.24, 0.34 and 0.39 in control, 0.5,
1.0, 2.0 and 5.0 ppm CdCl2
respectively.? In vivo
chromosome preparation from kidney tissues was carried out. The mean
frequency of cells with aberrations observed was 0.13, 0.20, 0.34, 0.60 and
0.95 in control, 0.5, 1.0, 2.0 and 5.0 ppm CdCl2 respectively. Likewise the mean
frequency of SCE observed was 0.05, 0.16, 0.36, 0.44 and 0.52 in control,
0.5, 1.0, 2.0 and 5.0 ppm CdCl2
respectively. It has been revealed from the results of this study that
cadmium produced genotoxic effects in fish. ?????
test, Chromosomal? aberrations, Chromatid exchange, Cadmium chloride ??
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