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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue May 2012, 33 (3)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Molecular typing of mealybug Phenacoccus solenopsis populations

from different hosts and locations in Punjab, India

 

Author Details

 

Satnam Singh

Punjab Agricultural University, Regional Station, Faridkot-151 203, India

Rakesh Sharma

Punjab Agricultural University, Regional Research Station, Ballowal Saunkhri-144 521, India

Rajinder Kumar

(Corresponding author)

Department of? Entomology, Punjab Agricultural University, Ludhiana -141 004, India ? ?????????

e-mail : rajinder9124@rediffmail.com

V. K. Gupta

Department of Entomology, Punjab Agricultural University, Ludhiana -141 004, India

V. K. Dilawari

Department of Entomology, Punjab Agricultural University, Ludhiana -141 004, India

 

 

Publication Data

Paper received:

26 June 2010

 

Revised received:

2? September 2011

 

Accepted:

20 September 2011

 

Abstract

True identity and existence of genetic variability in mealy bug from different regions holds immense significance for adopting appropriate control measures along with predicting the development of any biotypes. Mealy bug, Phenacoccus solenopsis adults were collected from four host plants i.e., Gossypium hirsutum ? cotton? (C), Abelmoschus esculentus ? okra (O), Pennisetum glaucum ? Napier Bajra? (B) and a weed ? Parthenium hysterophorus (P) in five cotton growing districts i.e., Abohar, Bathinda, Mansa, Muktsar and Faridkot of Punjab state. Variability among different populations was investigated through comparative analysis of four different RAPD markers. The genetic similarity dendrogram established that irrespective of the host plant and the collection site, 20 mealybug populations were distinguishable into two major clades that were related to each other by 68 %. Clade 1 included populations from Abohar district; it also included a single population each from Muktsar and Bathinda districts. The populations from all the other districts were grouped under Clade 2 with genetic similarity of 78 % Even under Clade 2, individual populations appeared to exist in location specific sub-clades. Thus, there is great possibility of development of biotypes which may differ in resistance to insecticides and host plant specificity. ?

 

Key words

Cotton, Genetic diversity, Mealy bug, Phenacoccus solenopsis?

 

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