Journal of Environmental Biology

Plasmid profiles of mercuric chloride tolerant rhizobia

from horse gram (Macrotyloma uniflorum)

Author Details

Prabhavati Edulamudi

(Corresponding author)

Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur - 522 510, India

e-mail: prabha_anumicro@rediffmail.com

Antony Johnson A. M.

Department of Virology, Sri Venkateswara University, Chittor, Tirupati - 517 502, India

Venkata Ramana Sai Gopal Divi

Department of Virology, Sri Venkateswara University, Chittor, Tirupati - 517 502, India

Veera Mallaiah Konada

Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur - 522 510, India

Publication Data

Paper received:

06 August 2010

Revised received:

03 March 2011

Accepted:

21 April 2011

Abstract

Thirty two rhizobia were isolated from the fresh healthy root nodules of horse gram. They were found to be highly salt tolerant. They were identified as rhizobia by cultural, biochemical and 16S rRNA sequence. The sequences of the four selected isolates were deposited in the NCBI GenBank. The obtained accession numbers were GQ483457, GQ483458, GQ483459 and GQ483460.  All the rhizobia were able to grow at 10 ppm mercuric chloride concentration. Four isolates HGR-11, 16, 30 and 31 were used to study the effect of different concentrations of mercuric chloride on the growth of rhizobia. These isolates were able to grow at 30 ppm concentration also. In these isolates, HGR-11 and HGR-30 showed maximum growth at 20 ppm than at control. These isolates contained one mega plasmid (~ 22 kb) at 20 ppm mercuric chloride concentration.

Key words

Metal tolerance, Mercuric chloride, Rhizobia, Plasmids, Macrotyloma uniflorum

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