nstantaneous and historical temperature effects on a-pinene
Plasmid profiles of mercuric
chloride tolerant rhizobia
from horse gram (Macrotylomauniflorum)
Author Details
PrabhavatiEdulamudi
(Corresponding author)
Department
of Botany and Microbiology, AcharyaNagarjunaUniversity, Guntur
- 522 510, India
e-mail:
prabha_anumicro@rediffmail.com
AntonyJohnson
A. M.
Department
of Virology, SriVenkateswaraUniversity, Chittor, Tirupati - 517 502, India
Venkata Ramana Sai Gopal Divi
Department
of Virology, SriVenkateswaraUniversity, Chittor, Tirupati - 517 502, India
VeeraMallaiahKonada
Department
of Botany and Microbiology, AcharyaNagarjunaUniversity, Guntur
- 522 510, India
Publication Data
Paper received:
06
August 2010
Revised
received:
03
March 2011
Accepted:
21 April 2011
Abstract
Thirty two rhizobia
were isolated from the fresh healthy root nodules of horse gram. They were
found to be highly salt tolerant. They were identified as rhizobia
by cultural, biochemical and 16S rRNA sequence. The
sequences of the four selected isolates were deposited in the NCBI GenBank. The obtained accession numbers were GQ483457,
GQ483458, GQ483459 and GQ483460.? All
the rhizobia were able to grow at 10 ppm mercuric chloride concentration. Four isolates
HGR-11, 16, 30 and 31 were used to study the effect of different
concentrations of mercuric chloride on the growth of rhizobia.
These isolates were able to grow at 30 ppm
concentration also. In these isolates, HGR-11 and HGR-30 showed maximum
growth at 20 ppm than at control. These isolates
contained one mega plasmid (~ 22 kb) at 20 ppm
mercuric chloride concentration.
Key words
Metal
tolerance, Mercuric chloride, Rhizobia, Plasmids, Macrotylomauniflorum
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