nstantaneous and historical temperature effects on a-pinene
Comparison of PCR based marker systems
for genetic
analysis in different cultivars of mango
Author Details
NavinSrivastava
(Corresponding author)
Division
of Crop Improvement, Central Institute for Subtropical Horticulture, Lucknow - 227 107, India
e-mail:
navinbiochem@gmail.com?
AnjuBajpai
Division
of Crop Improvement, Central Institute for Subtropical Horticulture, Lucknow - 227 107, India
RameshChandra
Division
of Crop Improvement, Central Institute for Subtropical Horticulture, Lucknow - 227 107, India
S. Rajan
Division
of Crop Improvement, Central Institute for Subtropical Horticulture, Lucknow - 227 107, India
M.Muthukumar
Division
of Crop Improvement, Central Institute for Subtropical Horticulture, Lucknow - 227 107, India
ManojKumarSrivastava
Department
of Biochemistry, BundelkhandUniversity, Jhansi - 284?
128, India
Publication Data
Paper received:
12
April 2010
Revised
received:
20
May 2011
Accepted:
14 June 2011
Abstract
Native diversity is well represented in northern and
eastern parts of India
for mango. We evaluated three important polymerase chain reaction (PCR) based
marker techniques viz., random amplified polymorphic DNA (RAPD), inter
simple sequence repeat (ISSR) and directed amplified mini satellite DNA
(DAMD) and examined their suitability for depicting genetic relationships and
discrimination among closely related group of 46 mango varieties grown in the
different agro-ecological zones in Uttar Pradesh, Bihar and West Bengal. Nine
RAPD, eleven ISSR and four DAMD primers generated 110, 160 and 43 discrete
fragments, respectively, accounting for polymorphism of 87.3, 79.83 and
83.72%, respectively. Cumulative analysis of these markers resulted in
comprehensive UPGMA based dendrogram where in
native mangoes representing important breeding lines and varieties from Uttar
Pradesh fall more or less in separate cluster, while Bihar and West Bengal
cultivars represent genetically different lineage forming distinct separate
cluster. The prime focus on the study was towards identification of genetic
variability that warrants establishing origin and molecular evolution of
mango cultivars of eastern and northern India because they are the rich
gene pool for conservation. Highest diversity index (DI) and polymorphic
information content (PIC) values were found in DAMD indicating it to be more
informative than others. Similarly, high effective multiplex ratio (EMR) and
marker index (MI) were recorded by ISSR reflecting ability to simultaneously
detect a large number of bands. The study accomplished establishing genetic
relationship and also DNA fingerprint development. The data is also useful
for mapping studies for gene identification.
Key words
Mango,
Genetic relationship, Polymorphic information content, Marker index,
Effective multiplex ratio, Diversity index
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