Journal of Environmental Biology
pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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Abstract - Issue Jan 2012, 33 (1) Back
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Optimization of solid state fermentation
conditions for the production of cellulase
by Trichoderma reesei Author Details |
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(Corresponding author) |
Department
of Biotechnology, e-mail:
devendrabiochem@gmail.com |
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Department
of Chemical Engineering, |
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Department
of Chemical Engineering, |
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Department
of Biotechnology, Madhav Institute of Technology
and Science, |
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Publication Data Paper received: 23
October 2009 Revised
received: 21
June 2010 Re-revised
received: 31
March 2011 Accepted: 14 June 2011 |
Abstract Cellulase production studies have been
carried out using the fungal strain Trichoderma
reesei NCIM 992 by using three different lignocellulosic materials by solid state fermentation
(SSF). The effect of basic fermentation parameters (pH, temperature, moisture content, particle size of substrate and
moistening agent) on enzyme production was studied. Maximum cellulase production was 2.63 U ml-1
using wheat bran as substrate. The optimal conditions for cellulase production for wheat bran were found to be:
initial moisture content-70%, initial medium pH-5.0, temperature-30oC,
moistening agents (MSS) and particle size of substrate (500 ?m).The optimal
incubation time for production was six days. Results indicate the scope for
further optimization of the production conditions to obtain higher cellulase titres using the
strain under SSF. Key words Cellulase, Trichoderma reesei, Solid state fermentation, Lignocellulosic
material, Wheat bran |
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