Journal of Environmental Biology

Production and characterization of carboxymethyl cellulase from

Paenibacillus polymyxa using mango peel as substrate

Author Details

Devendra Kumar

Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow - 227 107, India

Mohd. Ashfaque

Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow - 227 107, India

M. Muthukumar

Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow - 227 107, India

Munna Singh

Botany Department, Lucknow University, Lucknow - 226 007, India

Neelima Garg

(Corresponding author)

Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow - 227 107, India

e-mail: neelimagargg@rediffmail.com

Publication Data

Paper received:

28 August 2010

Revised received:

10 March 2011

Accepted:

21 April 2011

Abstract

Mango peel, a solid mango processing waste, comprises 15-20% of total fruit weight. This, being a rich source of lignocelluloses, was used as substrate for carboxymethyl cellulase (CMCase) production using Paenibacillus polymyxa. Maximum CMCase production (7.814 U mg^-1) was observed in a medium containing 7% mango peel (w/v) with 1.5% ammonium sulphate (w/v) at 37^oC and pH 5.5. Purification to an extent of 28.24 fold was achieved by affinity column chromatography. Bands corresponding to 26.5 and 34.0 kDa molecular sizes were observed on 12% denaturing Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) while of 72 kDa on 10% non- denaturing Native-PAGE, proving its heteromeric multienzyme nature. The enzyme was stable over a range of 20-60^oC and pH of 4.0-7.5. Michaelis-Menten equation constant (K_m and V_max) values of purified CMCase were 8.73 mg ml^-1 and 17.805 mM ml^-1 min^-1, respectively.

Key words

Carboxymethyl cellulase, Fruit waste, Mango peel, Paenibacillus polymyxa

Copyright ? 2012 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).