nstantaneous and historical temperature effects on a-pinene
Assessment of genetic
variation in lucerne (Medicago
sativa L.)
using protease inhibitor
activities and RAPD markers
Author Details
AmareshChandra
(Corresponding
author)
Crop Improvement Division, Indian Grassland and Fodder Research Institute,
Jhansi-284003, India
e-mail: amaresh62@yahoo.com
KrishnaChandra? Pandey??
Crop Improvement Division, Indian Grassland and Fodder Research
Institute,
Jhansi - 284
003, India
Publication Data
Paper received:
11 April 2009
Revised
received:
03 March 2010
Accepted:
19 April 2010
Abstract
The
aim of the presentstudy is to identify and characterize lucerne lines resistance to weevil infestation.Afterthree years of field screening for resistance to weevil infestation, 13lines of lucerne were selected to assess the
genotypic variations for lucerne weevil (HyperaposticaGyll.) at biochemical and molecular levels. Total phenols
varied from 0.15 to 0.91 mg g-1 (DM) in these genotypes. The
highest trypsin (11.11 unit mg-1
protein) and chymotrypsin (93.0 unit mg-1
protein) inhibitors activities were recorded in G-1-02 and B-4-03 lines
respectively, whereas highest a-amylases inhibitor activity
(14.2 unit mg-1 protein) in C-6-01. Zymogram
patterns for trypsin inhibitor activity showed quantitative
variations among the lines. In total 262 DNA fragments were generated when 45
deca-mer random primers were employed. Genetic
variation in terms of genetic distance ranged from 0.65 to 0.85. Sequential
Agglomerative Hierarchical and Nested (SAHN) clustering using the Un-weighted
Pair Group Method with Arithmetic mean (UPGMA) algorithmyielded two
clusters (cluster I and II) which converged at 72% similarity level. Cluster
I contained most of the lines having low level of weevil infestation. High bootstrap
values (>40) indicated the significance of nodes embodied in these two
clusters. However, SDS-PAGE analysis of the leaf proteins of these 13 lines
showed no major variations except minor difference in the protein bands of
molecular weights between 14 to 20 kD.
Copyright ? 2011 Triveni Enterprises. All rights reserved. No part
of the Journal can be reproduced in any form without prior
permission. Responsibility regarding the authenticity of the data, and
the acceptability of the conclusions enforced or derived, rest completely
with the author(s).