Journal of Environmental Biology

Active pharmaceutical ingredient (api) from an estuarine fungus,

Microdochium nivale (Fr.)

Author Details

S.H. Bhosale

Biochemical Sciences, National Chemical Laboratory, Pune - 411 008, India

K. B. Patil

Botany Dept., Shivaji Univ, Kolhapur - 416 105, India

P.S. Parameswaran

Bio-organic Group, Chemical Oceanography Division, National Institute of Oceanography,

Dona-Paula, Goa - 403 004, India

C.G. Naik

Bio-organic Group, Chemical Oceanography Division, National Institute of Oceanography,

Dona-Paula, Goa - 403 004, India

T.G. Jagtap

(Corresponding author)

Biological Oceanography Division, National Institute of Oceanography,

Dona-Paula, Goa - 403 004, India

e-mail: tanaji@nio.org

Publication Data

Paper received:

23 March 2010

Revised received:

08 October 2010

Re-revised received:

10 December 2010

Accepted:

08 January 2011

Abstract

Various marine habitats sustain variety of bio-sources of ecological and biotech potentials. Pharmaceutical potential compound Cyclosporine A was reported from marine fungus Microdochium nivale associated with Porteresia coarctata, a marine salt marsh grass from mangrove environment distributed along the Central West Coast (CWC) of India. This study involves association of M.? nivale with P. coarctata plant, fermentation conditions, purification of Cyclosporine A, chemical characterization etc. Its antifungal inhibition and MIC (Minimum inhibitory concentration) against Aspergillus strains (A. niger, A. japonicus, A. fresenii), yeasts and dermatophytes (Candida sp., Cryptococcus neoformans, Trichophyton mentagrophytes, T. tonsurans, T. violaceum, Microsporium gypsum and Fusarium sp.) were evaluated. However, the MIC against A. japonicus, C. neoformans, Candida sp. and T. tonsurans were confirmed to be as low as 12.5-25 mg disc^-1. The antifungal properties of Cyclosporine A against Aspergillus species, yeast and dermatophytes revealed that Cyclosporine A would be a potential compound for life threatening diseases caused by above fungi in both human and animals. Furthermore, we have reported herewith another source of Cyclosporin A derived from filamentous fungus, M.? nivale. occurring in marine environment.

Key words

Microdochium nivale, Cyclosporine A, Antifungal, Estuarine environment

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