nstantaneous and historical temperature effects on a-pinene
Effect of caffeine,
norfloxacin and nimesulide on heartbeat
and VEGF expression of
zebrafish larvae
Author Details
ChiranjibChakraborty
School of Bioscience
and Technology, VITUniversity, Vellore
- 632 014, India
C.H.Hsu
Department of Marine
Biotechnology and Resources, College
of Marine Science, NationalSunYat-senUniversity,
Kaohisung - 80424, Taiwan
Z.H. Wen
Department of Marine
Biotechnology and Resources, College
of Marine Science, NationalSunYat-senUniversity,
Kaohisung - 80424, Taiwan
C.S.Lin
Department of Marine
Biotechnology and Resources, College
of Marine Science, NationalSunYat-senUniversity,
Kaohisung - 80424, Taiwan
GovindasamyAgoramoorthy
(Corresponding
author)
Department of Pharmacy, TajenUniversity, Yanpu, Pingtung 907, Taiwan
e-mail:
agoram@mail.tajen.edu.tw
Publication Data
Paper received:
23 September 2009
Revised
received:
04 April 2010
Accepted:
05 May 2010
Abstract
The use of pharmaceuticals during pregnancy may causes
abnormalities to the embryo. Sometime the drug also effect to the new born if
the drug transferred through lactation. We have used zebrafish model to see
the effect of some pharmaceuticals on embryos and larvae. Three drugs,
caffeine, norfloxacin and nimesulide, were used for this study to see the
effect mainly the hatching rate of eggs, heart beat rate and the vascular
endothelial growth factor (VEGF) expression of the larvae. VEGF is an
important signaling protein that involved generating the new blood vessels
during embryonic development. We have used 10, 20, 50, 100 µg ml-1
concentrations of all the drugs to see the effect. No significant mortality
or malformations were observed in zebrafish embryos. Hatching was stared from
60 hr. In control group, 91% hatching rate was observed.Lowest hatching rate was observed using
highest concentration of norfloxacin (100 µg ml-1) and nimesulide
(100 µg ml-1) i.e. 55 and 56% respectively. In control group,
110 to 115 heart beat rate was counted per minute. Significantly higher heart
beat was observed in caffeine treated group which is 125 to140 min-1.
Lower heart beat was noted in nimesulide treated group which is 100 min-1.
We have tried to observe the possible effect of VEGF of the larvae by these
three drugs. Expression of VEGF was very low in caffeine treated group.
Almost no VGF expression was observe in 100 µg ml-1 caffeine
treated group. These studies suggest that there is a possibility that high
dosage of caffeine can harm the unborn baby or new born babies, if the
mothers use caffeine.