Sevil Toroglu
(Corresponding author)
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Department of Biology, Faculty of
Arts and Science, Kahramanmaras Sutcu Imam
University - 46100,
Kahramanmaras, Turkey
e-mail:
storoglu@ksu.edu.tr
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Publication Data
Paper received:
21
May 2009
Revised
received:
22
March 2010
Accepted:
20 April 2010
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Abstract
Spices and herbs have been used for many years by
different cultures. The aim of the present study is (1) to investigate
in-vitro antimicrobial effects of different spices and herbs (5 species: Rosmarinus officinalis
(Rosemary), Coriandrum
sativum (coriander), Micromeria
fruticosa (L.) Druce subsp. Brachycalyx P.H. Davis
(White micromeria), Cumium
cyminum (cumin), Mentha
piperita (Peppermint) against different
bacteria and fungi species, and (2) to discuss the in-vitro possible
effects between the plants and antibiotics. The microorganisms used were Micrococcus
luteus LA 2971, Bacillus megaterium
NRS, Bacillus brevis FMC 3, Enterococcus
faecalis ATCC 15753, Pseudomonas pyocyaneus DC 127, Mycobacterium smegmatis CCM 2067, Escherichia coli DM, Aeromonas hydrophila ATCC
7966, Yersinia enterocolitica
AU 19, Staphylococcus aureus Cowan 1, Streptococcus
faecalis DC 74 bacteria, and Saccharomyces cerevisiae
WET 136, Kluvyeromyces fragilis DC 98 fungi in this study. The results
indicated that essential oils of Rosmarinus
officinalis, Coriandrum
sativum L., Micromeria
fruticosa (L.) Druce subsp. brachycalyx P.H. Davis,
Cumium cyminum
L., Mentha piperita
L. were shown antimicrobial activity in the range of 7-60 mm 2 ?l-1 inhibition
zone to the microorganisms tested, using disc diffusion method. Standard
antibiotic such as Gentamicin (10 ?g), Cephalothin (30 ?g), Ceftriaxone
(10 ?g), Nystatin (10 U) discs were used for
comparison with the antimicrobial activities of essential oils of these
plants. In addition, antibacterial activity of essential oils of these plants
was researched by effects when it was used together with these standard
antibiotics in vitro. However, antibacterial activity changed also by in
vitro interactions between these standard antibiotics and essential oils
of these plants. Synergic, additive or antagonist effects were observed in
antibacterial activity.?
Key words
Spice,
Essential oils, Antibacterial and antifungal effects, Synergisms, Additive,
Antagonism
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