Journal of Environmental Biology

Impact of temperature on heat shock protein expression of Bombyx mori

cross-breed and effect on commercial traits

Author Details

J. Hongray Howrelia

P.G. and Research Department of Advanced Zoology and Biotechnology, Loyola College, Chennai - 600 034,

India

Bharat Bhusan Patnaik

(Corresponding author)

Central Sericultural Research and Training Institute, Central Silk Board (Govt. of India), Berhampove - 742 101,

India

e-mail: drbharatbhusan4@gmail.com

M. Selvanayagam

P.G. and Research Department of Advanced Zoology and Biotechnology, Loyola College, Chennai - 600 034,

India

S. Rajakumar

Regional Sericultural Research Station, Central Silk Board, Allikuttai (P.O.), Veeranam Road, Salem - 636 003,

India

Publication Data

Paper received:

22 October 2009

Revised received:

16 March 2010

Accepted:

15 July 2010

Abstract

The present study investigated the effect of increasing temperature stress on the thermotolerance of B. mori cross-breed PM x CSR₂ and tissue specific differential expression of heat shock proteins at IV^th and V^th instars. The larvae reared at 25 ? 1^oC and 70 ? 5% relative humidity were treated as control. Larvae were subjected to heat shock temperatures of 34, 38 and 42^oC for 3 hr followed by 3 hr recovery. Expression of Heat shock protein 72 were analyzed by SDS-PAGE and confirmed by western blotting analysis. The impact of heat shock on commercial traits of cocoons was analyzed by following different strategies in terms of acquired thermotolerance over control. Resistance to heat shock was increased as larval development proceeds and increased thermotolerance is achieved with the induction of Heat shock protein 72 in the V^th instar larval haemolymph. Relative influence of heat shock temperatures on commercial traits corresponding to the generation of heat shock protein 72 was significantly improved over control. In PM x CSR₂, cocoon and shell weight significantly increased to 9.90 and 11.90% over control respectively.

Key words

Commercial traits, Bombyx mori, Haemolymph, Heat shock protein, Temperature stress, Thermotolerance

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