Journal of Environmental Biology
pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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Abstract - Issue Jan 2011, 32 (1) Back
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Screening and detection of
biomarkers in chickpea plants exposed to chromium and
cadmium Author Details |
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Centre for Transgenic Plant
Development, Department of Biotechnology, |
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Mauji
Ram |
Centre
for Transgenic Plant Development, Department of Biotechnology, |
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Department
of Botany, |
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M. |
Centre for
Transgenic Plant Development, Department of Biotechnology, |
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Centre
for Transgenic Plant Development, Department of Biotechnology, |
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Kamaluddin |
Faculty
of Agriculture and Regional Research Station, Wadura, Sopore, Sher-e-Kashmir
University of Agriculture Science and Jammu Technology, and Kashmir, India |
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Centre
for Transgenic Plant Development, Department of Biotechnology, |
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Centre
for Transgenic Plant Development, Department of Biotechnology, |
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(Corresponding author) |
Centre for Transgenic Plant Development, Department of
Biotechnology, ? e-mail: mzabdin@rediffmail.com |
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Publication Data Paper received: 14 January 2009 Revised
received: 20 May 2010 Accepted: 01 June 2010 |
Abstract A broad screening protocol,
covering the most general phytochemical groups of compounds, was developed on
the basis of high performance thin layer chromatography (HPTLC). A total of
six TLC systems, comprising three derivatization reagents, two stationary
phases and two mobile phases, were included. The screening method was applied
for the identification of biomarkers in the chickpea plant exposed to cadmium
and chromium. The biomarkers were selected on the basis of significant
changes (0.26-4.6 fold) in concentration levels of phytochemicals. Totally,
five different amino acids, three organic acids, one sulphur containing
compound and one sugar were identified as biomarkers in chickpea exposed
heavy metal. Key
words Screening, Biomarker,
Phytochemical, Chickpea, Thin layer chromatography |
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