Antagonistic
and plant growth activity of Trichoderma
isolates of Western Himalayas
B.B. Joshi*1, R.P. Bhatt2 and D. Bahukhandi3
?
1Crop Protection Division, Indian Institute of Sugarcane Research, Lucknow - 226 002, India
2Department of Botany, HNB, Garhwal University,
Srinagar Pauri
Garhwal
- 246 174, India
3Division of Seed Technology, India Grassland and Fodder Research Institute (IGFRI),
Jhansi - 284 003, India
(Received: September 23, 2009; Revised
received: February
24, 2010; Accepted: February 30, 2010)
Abstract: The genus Trichoderma
is rapidly growing colonies bearing tufted or postulate, repeatedly branched
conidiophores with lageniform phialides
and hyaline or green conidia born in slimy heads. 62 isolates of Trichoderma species were isolated from different rhizospheric soil samples collected from different places
located in Western Himalayas region. Out of
these only two species were found i.e. Trichoderma hazianum and Trichoderma viride. Their efficacy against soil borne plant pathogens
like Sclerotium rolfsii, Rhizoctonia solani and Sclerotinia sclerotiorum revealed
that only three isolates amounting to 5% of the total collected isolates of
this region were found highly antagonist. Among them 5% isolates were found
against S. rolfsii, 13% isolates against? R. solani,
10% against sclerotium caused above 80% inhibition of
mycelial growth respectively. 6% isolates out of
twenty seven utilized chitin by more than 80 and 16% isolates consumed
cellulose by above 80% and therefore are producers of chitinase
and cellulases. 58% isolates produced colonies having
cottony texture and 41% produced dark green colonies. Pigmentation as observed
from reverse side of the colony revealed that 70% of them did not produced
pigment in the medium. Plant growth promotion measured as root and shoot
lengths were significantly higher than in control. The maximum root length and
shoot length were recorded when seeds were treated with isolates were recorded
at Srinagar Garhwal was
4.70 and 4.75 cm out of all the isolates in which isolate recorded from Srinagar no 3 caused maximum percent seed germination which
was significantly higher 79.49%.
Key
words:? Trichoderma, Isolates, Antagonist
potential, Mycelial growth, Chitinase, Cellulose, Plant pathogen
PDF of full length paper is available
online
Copyright ? 2010 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any
form without prior permission. Responsibility regarding the authenticity
of the data, and the acceptability of the conclusions enforced or derived, rest
completely with the author(s).
