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Journal of Environmental BiologypISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP |
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Abstract - Issue Sep 2010, 31 (5) BackCytotoxicity assessment of monocrotophos in Paramecium
caudatum
and Oxytricha fallax Protozoology and
Environmental Toxicology Lab., Department of Zoology, (Received: Abstract: Experiments were conducted to evaluate
the toxic effects of monocrotophos in ciliate models
Paramecium caudatum and Oxytricha
fallax. In acute toxicity studies higher
concentrations of monocrotophos caused marked
increase in mobility of cells exhibiting rocking movements within two mins of exposure but were decreased after 30 mins. LC50 value by mortality curve for 3 hr
acute toxicity test of Oxytricha fallax
and Paramecium caudatum was found 307.744?33.27mg l-1
and 332.284?57.52 mg l-1 respectively. Oxytricha
fallax was found sensitive than Paramecium caudatum to monocrotophos. In
acute exposure cells showed deformities such as swelling, oval shaped deformity
and in higher concentrations shortening of longitudinal axis with blackening of
cytoplasm occurred. The length of paramecia was reduced prominently. Similarly,
enlargement of contractile vacuole and stress egestion
of food vacuoles was also observed. The morphological studies showed the
changes in shape, size, colour and width of Paramecia
and Oxytricha. Frequencies of macronuclear
aberrations were significant showing deformities such as rod shaped,
elongation, fragmentation, diffusion and total absence of nucleus and were
concentration dependent. The data provided in the present study, on interaction
of pesticides with nuclear structure can be of immense value because most of
these pesticides have been reported to have carcinogenic, mutagenic and teratogenic properties. Key
words: Monocrotophos, Paramecium caudatum, Oxytricha
fallax, Acute toxicity, Behavioural responses, Macronuclear
aberrations ??????? PDF of full length paper
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