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Journal of Environmental BiologypISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP |
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Abstract - Issue Sep 2010, 31 (5) BackImpact of osmotic stress on
seed germination and seedling growth
in black gram (Phaseolus mungo) Veer Pratap2 and 1Department of Botany, 2P.M.S.
(Received: Abstract: An experiment was conducted to see the
impact of osmotic stress as it is one of the main cause
in various soil and water disorders in agricultural field crops, specially the
seed germination and seedling growth. The osmotic stress was generated using
PEG-6000 and the seed germination, seedling growth were evaluated including the
status of pigments i.e. chlorophyll (a, b and total), total carotenoids,
pheophytin (a, b and total) and different enzymes
like amylase, peroxidase, catalase
and superoxide dismutase.
The various osmotic potentials generated (-2, -5 and -10 bars) showed
significant decrease in germination percentage as at the osmotic potential of
-10 bars it was observed 70 in comparison to 90% of control. All the seedling
growth parameter also showed inhibition with increase in osmotic potential.
Increase in osmotic stress decreased Chlorophyll ?a?, while Chlorophyll ?b? was
increased in -5 bars while total chlorophyll showed decrease in -5 bars osmotic
potential. Total carotenoids and pheophytin
(a, b and total) were highly increased in -5 bars and decreased in -10 bars
osmotic concentration. Enzymatic activity was found to be decreased in amylase
while peroixidase, catalase
and SOD were increased at different osmotic gradients in comparison to control.
The data observed in the experiment can be helpful to assess the impact of any
kind of osmotic stress on plant growth and development in crops. Key
words: PEG-6000, Osmotic potential, Seed
germination, Seedling growth, Phaseolus mungo ??????? PDF of full length paper
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