JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Home
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
 

Google Search the Journal web-site:


    Abstract - Issue Sep 2010, 31 (5)                                     Back


abstract_01

Diversification of nitrogen fixing bacterial community using nifH gene as a

biomarker in different geographical soils of Western Indian Himalayas

 

Chhug Singh, Ravindra Soni, Sourabh Jain, Subhadip Roy and Reeta Goel*

 

Department of Microbiology, CBSH, G.B. Pant University of Agriculture and Technology, Pantnagar - 263 145, India

(Received: May 13, 2009; Revised received: September 02, 2009; Accepted: September 23, 2009)

 

Abstract: Six soil samples (Pantnagar, Chamoli, Almora, Ranichauri, Pithoragarh and Badrinath) belonging to different geographical locations of Western Himalayas in India, were analyzed to diversify the nitrogen fixing bacterial community using nifH gene biomarker. DNA from soil samples were isolated and amplified using nifH gene specific primers. Genomic DNA and PCR amplified products were then individually subjected to restriction digestion with tetra to octacutter enzymes (AluI, MspI, BglII, XbaI, HindIII, HaeIII, AluI, MspI and PasI. Further, restriction pattern was studied by preparing dendograms on the basis of similarity matrix and compared for the nifH community.? It was observed that temperate region soils (Ranichauri and Pithoragarh) were negative for nifH marker while subalpine region (Badrinath) and tarai region soils (Pantnagar) documented similar nifH community. Moreover, the direct genomic DNA restriction analysis indicated that subalpine region soil (Badrinath) was most diversified.

Key words: Soil sample, Geographical regions, Diversity, RFLP, NifH

?????? PDF of full length paper is available online

 

 

Copyright ? 2010 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).