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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Jul 2010, 31 (4)                                                                                                             Back



Abstract _5

Induction of oxidative stress by non-lethal dose of mercury in rat liver:

Possible relationships between apoptosis and necrosis

 

Bharat Bhusan Patnaik*1, Atish Roy2, Soumik Agarwal2 and Shelley Bhattacharya2

1Department of Biotechnology, Iase University (Off Campus Centre), Mancheswar Industrial Estate, Bhubaneswar - 751010, India

2Environmental Toxicology Laboratory, Department of Zoology, Visva-Bharathi University, Santiniketan - 731235, India

(Received: November 19, 2008; Revised received: July 20, 2009; Accepted: August 07, 2009)

 

Abstract: Sprague Dawley strain of male rats weighing 200 ? 10.0 g, were exposed intramuscularly to non-lethal dose of mercury for short acute duration of 24 and 48 hr. Mercury treatment increased thio-barbituric acid reactive substance (TBARS) and conjugated diene (CD) content with increase in duration when compared with control. This reflects possible increase in lipid peroxidation, revealing that sufficient intoxication was generated by non-lethal dose of mercury. Furthermore, mercury treatment decreased tissue glutathione (GSH) content to 2.07 and 1.49 ?g GSH mg protein-1 with concomitant decrease in glutathione-S-transferase (GST) activity by 26.06 and 36.40% after 24 and 48hr of exposure respectively. The elevations of aspartate transaminase (AST) and alanine transaminase (ALT) levels measured exhibited increase of 287.5 and 214.5% after 48 hr of exposure respectively which were found to be highly significant compared with control. Western blot analysis indicated upregulation of caspase-9 and upsurge in effector caspase-3 activity leading to apoptosis. The concluded findings of the present investigation suggests? possible role of early mercury exposure in inducing oxidative stress mediated apoptosis in mammalian model systems as an indicator component of environmental toxicology.

Keywords: Mercury, Oxidative stress, Lipid peroxidation, Glutathione, Apoptosis

???????? PDF of full length paper is available online

 

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