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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Nov 2009, 30 (6)                                     Back


Abstract_04

Synthesis and in vitro evaluation of 2,3-dibromo-3-methyl-1-phenylphospholane

1-oxide for potential anti-proliferative effects

 

Michio Fujie1,2, Satoki Nakamura3, Kazuhide Asai1, Junko Yamashita4, Keita Kiyofuji4 and Mitsuji Yamashita*4

 

1Departament of Materials Science, Graduate School of Science and Engineering, Shizuoka University, 3-5-1 Johoku,

Hamamatsu City, Shizuoka Prefecture - 432-8011, Japan

2Research Equipment Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu City,

Shizuoka Prefecture - 431-3192, Japan

3Department of Internal Medicine III, Hamamatsu University School of Medicine, 1-20-1 Handayama,

Hamamatsu City, Shizuoka Prefecture - 431-3192, Japan

4Department of Nano Materials, Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku,

Hamamatsu City, Shizuoka Prefecture - 432-8011, Japan

(Received: May 09, 2008; Revised received: October 20, 2008; Accepted: November 21, 2008)

 

Abstract: A novel phospha sugar analogue, 2,3-dibromo-3-methyl-1-phenylphospholane 1-oxide (DBMPP), was prepared from 1-phenyl-3-methyl-2- phospholene 1-oxide and evaluated by in vitro MTT method for leukemia cells and microscopic observations for solid tumor cells, e.g., stomach cancer cells.? The evaluation revealed clearly that the synthesized phospha sugar analogue DBMPP has competent potentials and excellent anti-cancer activities that killed selectively and specifically the leukemia cells of cell lines of K562 and U937 but did not give any damages on healthy leukocyte.? Moreover, it was revealed that DBMPP killed solid cancer cells such as stomach cancer cells and melanoma of cell lines of MKN45 and G361.? Therefore, DBMPP should exert anti-proliferative effects for different kinds of tumor cells based on the in vitro evaluations.? The cell cycle analyses by flow cytometry for K562 and U937 cells clearly demonstrated that the mechanism of the anti-proliferative effect on the human tumor cells is apoptosis induced by DBMPP.

Key words: Human tumor cell lines, 3-(4,5-Dimethythiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Apoptosis, DBMPP, Phospha sugars, Carcinostatic drug

???????????? PDF of full length paper is available online

 

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