Journal of Environmental Biology

Effect of seabuckthorn extract on delayed chlorophyll  fluorescence

on Cd and Co ions treated wheat seedlings

 

R.A. Ganiyeva¹, E.M. Novruzov², S.A. Bayramova¹, I.M. Kurbanova¹ and R.A. Hasanov*^1,3

¹Biophysics laboratory, ²Plant Resources Department, Institute of Botany, National Acadamy of Science, Baku-AZ 1073, Azerbaijan

³Department of Biophysics and Molecular Biology, Baku State University, Baku-AZ1148, Azerbaijan

(Received: June 23, 2008; Revised received: November 25, 2008; Accepted: November 25, 2008)

 

Abstract: The protecting effect of “Hypporamine PL” compound isolated from dry leaves of seabuckthorn (Hippophae rhamneides L.) on photosystem 2 (PS 2) activity suppression induced by CdCl₂ and CoCl₂ treatment in the 7 day old wheat seedlings (Triticum aestivum L.) under different pH of growth medium was investigated by measurement of millisecond delayed fluorescence (ms-DF) of chlorophyll intact leaves. The value o-i/p-s of ms-DF ratio was reduced under the Cd²⁺ and Co²⁺ treatments on 60 and 65% respectively at pH 6.7. Acidification of medium (pH 5.0) results in decreasing of ratio o-i/p-s only approximately on 30% in average. In the alkaline medium the lowering of o-i/p-s on 41% is observed in both ions treatments. This decreasing of o-i/p-s ratio occurred due to decreasing of fast phase o-i amplitude. At the same time the widening and increasing of slow phase p-s amplitude was observed. The compound “Hypporamine PL” limited the decrease of ms-DF components induced by heavy metals. It is suggested that the protective effect of “Hypporamine PL” on the photochemical reactions in the PS2 is due to catechins, epicatechins, quercetin  and other polyphenols, containing in this compound, preventing the free radicals formation in the PS2 under treatment by heavy metal ions.

Key words: Heavy metal ions, Seabuckthorn extract, Delayed fluorescence, Photosystem 2

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